Recombinant Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-PSMB10/MECL1 antibody [EPR14902]
See all PSMB10/MECL1 primary antibodies -
Description
Rabbit monoclonal [EPR14902] to PSMB10/MECL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PSMB10/MECL1 aa 50-150. The exact sequence is proprietary.
Database link: P40306 -
Positive control
- HuT 78, Raji, Human fetal spleen, Human fetal thymus; Human kidney adenocarcinoma tissue; Daudi cells.
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General notes
This product was previously labelled as PSMB10
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14902 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab183506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa). | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. -
Sequence similarities
Belongs to the peptidase T1B family. -
Developmental stage
Highly expressed in immature dendritic cells (at protein level). -
Post-translational
modificationsAutocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5699 Human
- Entrez Gene: 19171 Mouse
- Entrez Gene: 291983 Rat
- Omim: 176847 Human
- SwissProt: P40306 Human
- SwissProt: O35955 Mouse
- SwissProt: Q4KM35 Rat
- Unigene: 9661 Human
see all -
Alternative names
- beta2i antibody
- FLJ00366 antibody
- LMP10 antibody
see all
Images
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![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.com/ps/products/183/ab183506/Images/ab183506-215615-anti-psmb10-antibody-epr14902-western-blot.jpg)
Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution
Lane 1 : Human fetal spleen tissue lysate
Lane 2 : Human fetal thymus tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 29 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.com/ps/products/183/ab183506/Images/ab183506-215616-anti-psmb10-antibody-epr14902-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)Immunohistochemical analysis of paraffin-embedded Human Kidney adenocarcinoma tissue labeling PSMB10/MECL1 with ab183506 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.com/ps/products/183/ab183506/Images/ab183506-215618-anti-psmb10-antibody-epr14902-flow-cytometry.jpg)
Flow Cytometry - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)Flow cytometric analysis of Daudi cells (2% paraformaldehyde-fixed) labeling PSMB10/MECL1 with ab183506 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.com/ps/products/183/ab183506/Images/ab183506-215614-anti-psmb10-antibody-epr14902-western-blot.jpg)
Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/5000 dilution
Lane 1 : HuT 78 cell line lysate
Lane 2 : Raji cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 29 kDa
References
This product has been referenced in:
- Chen C et al. Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload. Redox Biol 20:390-401 (2019). Read more (PubMed: 30412827) »
- Textor A et al. Preventing tumor escape by targeting a post-proteasomal trimming independent epitope. J Exp Med 213:2333-2348 (2016). Read more (PubMed: 27697836) »
