Recombinant Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.com/ps/products/183/ab183506/Images/ab183506-393598-anti-psmb10mecl1-antibody-epr14902-western-blot-human-knockout-lysate.jpg "Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14902] to PSMB10/MECL1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PSMB10/MECL1 antibody [EPR14902]
See all PSMB10/MECL1 primary antibodies -
Description
Rabbit monoclonal [EPR14902] to PSMB10/MECL1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Daudi, HuT 78 and Raji cell lysates; Human fetal spleen and thymus tissue lysates. IHC-P: Human kidney adenocarcinoma tissue. Flow Cyt (intra): Daudi cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14902 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/90.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa).
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa). |
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. -
Sequence similarities
Belongs to the peptidase T1B family. -
Developmental stage
Highly expressed in immature dendritic cells (at protein level). -
Post-translational
modificationsAutocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5699 Human
- Entrez Gene: 19171 Mouse
- Entrez Gene: 291983 Rat
- Omim: 176847 Human
- SwissProt: P40306 Human
- SwissProt: O35955 Mouse
- SwissProt: Q4KM35 Rat
- Unigene: 9661 Human
see all -
Alternative names
- beta2i antibody
- FLJ00366 antibody
- LMP10 antibody
see all
Images
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Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PSMB10 knockout HeLa cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaLanes 1-4: Merged signal (red and green). Green - ab183506 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab183506 Anti-PSMB10/MECL1 antibody [EPR14902] was shown to specifically react with PSMB10/MECL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264923 (knockout cell lysate ab258145) was used. Wild-type and PSMB10/MECL1 knockout samples were subjected to SDS-PAGE. ab183506 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.com/ps/products/183/ab183506/Images/ab183506-215616-anti-psmb10-antibody-epr14902-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)Immunohistochemical analysis of paraffin-embedded Human Kidney adenocarcinoma tissue labeling PSMB10/MECL1 with ab183506 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.com/ps/products/183/ab183506/Images/ab183506-215618-anti-psmb10-antibody-epr14902-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)Intracellular flow cytometric analysis of Daudi cells (2% paraformaldehyde-fixed) labeling PSMB10/MECL1 with ab183506 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.com/ps/products/183/ab183506/Images/ab183506-215615-anti-psmb10-antibody-epr14902-western-blot.jpg)
Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/1000 dilution
Lane 1 : Human fetal spleen tissue lysate
Lane 2 : Human fetal thymus tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 29 kDa -
![Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.com/ps/products/183/ab183506/Images/ab183506-215614-anti-psmb10-antibody-epr14902-western-blot.jpg)
Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)All lanes : Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506) at 1/5000 dilution
Lane 1 : HuT 78 cell line lysate
Lane 2 : Raji cell line lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 29 kDa -
![Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)](https://www.abcam.com/ps/products/183/ab183506/Images/ab183506-6-benefits-of-recombinant-antibodies.png)
Anti-PSMB10/MECL1 antibody [EPR14902] (ab183506)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (8)
ab183506 has been referenced in 8 publications.
- Khilji MS et al. The intermediate proteasome is constitutively expressed in pancreatic beta cells and upregulated by stimulatory, low concentrations of interleukin 1 ß. PLoS One 15:e0222432 (2020). PubMed: 32053590
- Liao J et al. Deficiency of LMP10 Attenuates Diet-Induced Atherosclerosis by Inhibiting Macrophage Polarization and Inflammation in Apolipoprotein E Deficient Mice. Front Cell Dev Biol 8:592048 (2020). PubMed: 33195259
- Du SH et al. Co-Inhibition of the Immunoproteasome Subunits LMP2 and LMP7 Ameliorates Immune Thrombocytopenia. Front Immunol 11:603278 (2020). PubMed: 33552061
- Khilji MS et al. The inducible ß5i proteasome subunit contributes to proinsulin degradation in GRP94-deficient ß-cells and is overexpressed in type 2 diabetes pancreatic islets. Am J Physiol Endocrinol Metab 318:E892-E900 (2020). PubMed: 32255680
- Deng S et al. Host cell protein PSMB10 interacts with viral NS3 protein and inhibits the growth of classical swine fever virus. Virology 537:74-83 (2019). PubMed: 31493657
- Chen C et al. Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload. Redox Biol 20:390-401 (2019). PubMed: 30412827
- Huo X et al. Clinical and Expression Significance of AKT1 by Co-expression Network Analysis in Endometrial Cancer. Front Oncol 9:1147 (2019). PubMed: 31781484
- Textor A et al. Preventing tumor escape by targeting a post-proteasomal trimming independent epitope. J Exp Med 213:2333-2348 (2016). PubMed: 27697836