Recombinant Anti-PSMB10/MECL1 antibody [EPR14902] - BSA and Azide free (ab240272)
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Overview
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Product name
Anti-PSMB10/MECL1 antibody [EPR14902] - BSA and Azide free
See all PSMB10/MECL1 primary antibodies -
Description
Rabbit monoclonal [EPR14902] to PSMB10/MECL1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PSMB10/MECL1 aa 50-150. The exact sequence is proprietary.
Database link: P40306 -
General notes
ab240272 is the carrier-free version of ab183506 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab240272 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as PSMB10
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14902 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab240272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 29 kDa). | |
| Flow Cyt | Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. -
Sequence similarities
Belongs to the peptidase T1B family. -
Developmental stage
Highly expressed in immature dendritic cells (at protein level). -
Post-translational
modificationsAutocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5699 Human
- Entrez Gene: 19171 Mouse
- Entrez Gene: 291983 Rat
- Omim: 176847 Human
- SwissProt: P40306 Human
- SwissProt: O35955 Mouse
- SwissProt: Q4KM35 Rat
- Unigene: 9661 Human
see all -
Alternative names
- beta2i antibody
- FLJ00366 antibody
- LMP10 antibody
see all
Images
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![Flow Cytometry - Anti-PSMB10/MECL1 antibody [EPR14902] - BSA and Azide free (ab240272)](https://www.abcam.com/ps/products/240/ab240272/Images/ab240272-2-ab183506215618ab183506FC.JPG)
Flow Cytometry - Anti-PSMB10/MECL1 antibody [EPR14902] - BSA and Azide free (ab240272)Flow cytometric analysis of Daudi cells (2% paraformaldehyde-fixed) labeling PSMB10/MECL1 with ab183506 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183506).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] - BSA and Azide free (ab240272)](https://www.abcam.com/ps/products/240/ab240272/Images/ab240272-1-ab183506215616ab183506IHC.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB10/MECL1 antibody [EPR14902] - BSA and Azide free (ab240272)Immunohistochemical analysis of paraffin-embedded Human Kidney adenocarcinoma tissue labeling PSMB10/MECL1 with ab183506 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183506).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Datasheets and documents
References
ab240272 has not yet been referenced specifically in any publications.
