Recombinant
RabMAb

Recombinant Anti-PSMB10/MECL1 antibody [EPR14903] (ab190790)

Overview

  • Product name

    Anti-PSMB10/MECL1 antibody [EPR14903]
    See all PSMB10/MECL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14903] to PSMB10/MECL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human PSMB10/MECL1 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P40306

  • Positive control

    • Human fetal thymus lysate; Daudi and Raji cell lysates; HeLa cells.
  • General notes

     

     

     This product was previously labelled as PSMB10

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab190790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ICC/IF 1/100 - 1/250.

Target

  • Function

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
  • Sequence similarities

    Belongs to the peptidase T1B family.
  • Developmental stage

    Highly expressed in immature dendritic cells (at protein level).
  • Post-translational
    modifications

    Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • beta2i antibody
    • FLJ00366 antibody
    • LMP10 antibody
    • Low molecular mass protein 10 antibody
    • Macropain subunit MECl 1 antibody
    • Macropain subunit MECl-1 antibody
    • MECL1 antibody
    • MGC1665 antibody
    • Multicatalytic endopeptidase complex subunit MECl 1 antibody
    • Multicatalytic endopeptidase complex subunit MECl-1 antibody
    • OTTHUMP00000174858 antibody
    • Proteasome (prosome macropain) subunit beta type 10 antibody
    • Proteasome beta 10 subunit antibody
    • Proteasome catalytic subunit 2i antibody
    • Proteasome MECl 1 antibody
    • Proteasome MECl-1 antibody
    • Proteasome subunit beta 2i antibody
    • Proteasome subunit beta 7i antibody
    • Proteasome subunit beta type 10 antibody
    • Proteasome subunit beta type-10 antibody
    • Proteasome subunit beta-2i antibody
    • Proteasome subunit MECL1 antibody
    • PSB10_HUMAN antibody
    • PSMB 10 antibody
    • Psmb10 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PSMB10/MECL1 with ab190790 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor555) secondary antibody at 1/200 dilution. Counter stained with DAPI.

  • All lanes : Anti-PSMB10/MECL1 antibody [EPR14903] (ab190790) at 1/1000 dilution

    Lane 1 : Human fetal thymus lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

References

This product has been referenced in:

  • Norris EL  et al. Proteoform-Specific Insights into Cellular Proteome Regulation. Mol Cell Proteomics 15:3297-3320 (2016). WB ; Human . Read more (PubMed: 27451424) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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