Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14903] to PSMB10/MECL1
- Suitable for: WB, ICC/IF
- Reacts with: Human
Product nameAnti-PSMB10/MECL1 antibody [EPR14903]
See all PSMB10/MECL1 primary antibodies
DescriptionRabbit monoclonal [EPR14903] to PSMB10/MECL1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
within Human PSMB10/MECL1 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: P40306
- Human fetal thymus lysate; Daudi and Raji cell lysates; HeLa cells.
This product was previously labelled as PSMB10
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab190790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).|
|ICC/IF||1/100 - 1/250.|
FunctionThe proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
Sequence similaritiesBelongs to the peptidase T1B family.
Developmental stageHighly expressed in immature dendritic cells (at protein level).
modificationsAutocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- beta2i antibody
- FLJ00366 antibody
- LMP10 antibody
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PSMB10/MECL1 with ab190790 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor555) secondary antibody at 1/200 dilution. Counter stained with DAPI.
All lanes : Anti-PSMB10/MECL1 antibody [EPR14903] (ab190790) at 1/1000 dilution
Lane 1 : Human fetal thymus lysate
Lane 2 : Daudi cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
ab190790 has been referenced in 1 publication.
- Norris EL et al. Proteoform-Specific Insights into Cellular Proteome Regulation. Mol Cell Proteomics 15:3297-3320 (2016). WB ; Human . PubMed: 27451424