Anti-PSMB4 antibody (ab166792)
Key features and details
- Goat polyclonal to PSMB4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PSMB4 antibody
See all PSMB4 primary antibodies -
Description
Goat polyclonal to PSMB4 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Hamster, Cow, Dog, Pig, Monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to Human PSMB4 aa 233-243 (internal sequence) (Cysteine residue).
Sequence:QIATVTEKGVE
Database link: P28070 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 29 kDa).
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IHC-P |
Use a concentration of 3.75 µg/ml.
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Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 29 kDa). |
IHC-P
Use a concentration of 3.75 µg/ml. |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal macrophage proteasome (By similarity). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
Sequence similarities
Belongs to the peptidase T1B family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 506203 Cow
- Entrez Gene: 475848 Dog
- Entrez Gene: 101128397 Gorilla
- Entrez Gene: 5692 Human
- Entrez Gene: 19172 Mouse
- Entrez Gene: 100153923 Pig
- Entrez Gene: 100328673 Rabbit
- Entrez Gene: 58854 Rat
see all -
Alternative names
- 26 kDa prosomal protein antibody
- HN3 antibody
- HsBPROS26 antibody
see all
Images
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Anti-PSMB4 antibody (ab166792) at 0.3 µg/ml + HEK293 cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Primary incubation: 1 hour. -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling PSMB4 with ab166792 at 3.75 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab166792 has been referenced in 2 publications.
- Ulicna L et al. Multiple Aspects of PIP2 Involvement in C. elegans Gametogenesis. Int J Mol Sci 19:N/A (2018). PubMed: 30201859
- Pickering AM et al. Lifespan of mice and primates correlates with immunoproteasome expression. J Clin Invest 125:2059-68 (2015). PubMed: 25866968