Recombinant Anti-PSMB4 antibody [EPR9396] (ab137067)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB4 antibody [EPR9396] (ab137067)](https://www.abcam.com/ps/products/137/ab137067/Images/ab137067-151597-anti-proteasome-subunit-beta-type-4-antibody-epr9396-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB4 antibody [EPR9396] (ab137067)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9396] to PSMB4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PSMB4 antibody [EPR9396]
See all PSMB4 primary antibodies -
Description
Rabbit monoclonal [EPR9396] to PSMB4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PSMB4 aa 50-150. The exact sequence is proprietary.
Database link: P28070 -
Positive control
- HeLa, 293T, HepG2, and A431 cell lysates; Human colon tissue.
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General notes
This product was previously labelled as Proteasome subunit beta type 4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9396 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 29 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal macrophage proteasome (By similarity). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
Sequence similarities
Belongs to the peptidase T1B family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5692 Human
- Entrez Gene: 19172 Mouse
- Entrez Gene: 58854 Rat
- Omim: 602177 Human
- SwissProt: P28070 Human
- SwissProt: P99026 Mouse
- SwissProt: P34067 Rat
- Unigene: 89545 Human
see all -
Alternative names
- 26 kDa prosomal protein antibody
- HN3 antibody
- HsBPROS26 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB4 antibody [EPR9396] (ab137067)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling PSMB4 with ab137067 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-PSMB4 antibody [EPR9396] (ab137067)](https://www.abcam.com/ps/products/137/ab137067/Images/ab137067-151598-anti-proteasome-subunit-beta-type-4-antibody-epr9396-western-blot.jpg)
Western blot - Anti-PSMB4 antibody [EPR9396] (ab137067)All lanes : Anti-PSMB4 antibody [EPR9396] (ab137067) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa -
![Anti-PSMB4 antibody [EPR9396] (ab137067)](https://www.abcam.com/ps/products/137/ab137067/Images/ab137067-3-benefits-of-recombinant-antibodies.png)
Anti-PSMB4 antibody [EPR9396] (ab137067)
Protocols
Datasheets and documents
References (1)
ab137067 has been referenced in 1 publication.
- Cheng YC et al. Interference with PSMB4 Expression Exerts an Anti-Tumor Effect by Decreasing the Invasion and Proliferation of Human Glioblastoma Cells. Cell Physiol Biochem 45:819-831 (2018). PubMed: 29414809
