Recombinant
RabMAb

Recombinant Anti-PSMB4 antibody [EPR9396] (ab137067)

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Overview

  • Product name

    Anti-PSMB4 antibody [EPR9396]
    See all PSMB4 primary antibodies

  • Description

    Rabbit monoclonal [EPR9396] to PSMB4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PSMB4 aa 50-150. The exact sequence is proprietary.
    Database link: P28070

  • Positive control

    • HeLa, 293T, HepG2, and A431 cell lysates; Human colon tissue.

  • General notes

     

     

     This product was previously labelled as Proteasome subunit beta type 4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9396

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal macrophage proteasome (By similarity). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.

    • Sequence similarities

      Belongs to the peptidase T1B family.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 26 kDa prosomal protein antibody
      • HN3 antibody
      • HsBPROS26 antibody
      • HSN3 antibody
      • Macropain beta chain antibody
      • Multicatalytic endopeptidase complex beta chain antibody
      • PROS-26 antibody
      • PROS26 antibody
      • Proteasome (prosome macropain) subunit beta type 4 antibody
      • Proteasome beta 4 subunit antibody
      • Proteasome beta chain antibody
      • Proteasome chain 3 antibody
      • Proteasome subunit beta 4 antibody
      • Proteasome subunit beta type 4 antibody
      • Proteasome subunit beta type-4 antibody
      • Proteasome subunit HsN3 antibody
      • PSB4_HUMAN antibody
      • PSMB 4 antibody
      • PSMB4 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB4 antibody [EPR9396] (ab137067)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMB4 antibody [EPR9396] (ab137067)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling PSMB4 with ab137067 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-PSMB4 antibody [EPR9396] (ab137067)
      Western blot - Anti-PSMB4 antibody [EPR9396] (ab137067)
      All lanes : Anti-PSMB4 antibody [EPR9396] (ab137067) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    References

    This product has been referenced in:

    • Cheng YC  et al. Interference with PSMB4 Expression Exerts an Anti-Tumor Effect by Decreasing the Invasion and Proliferation of Human Glioblastoma Cells. Cell Physiol Biochem 45:819-831 (2018). Read more (PubMed: 29414809) »
    See 1 Publication for this product

    Publishing research using ab137067? Please let us know so that we can cite the reference in this datasheet.

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