Overview

  • Product name

    Anti-PSMB4 antibody [EPR9397]
    See all PSMB4 primary antibodies
  • Description

    Rabbit monoclonal [EPR9397] to PSMB4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PSMB4 aa 200-300. The exact sequence is proprietary.
    Database link: P28070

  • Positive control

    • 293T, HeLa and HepG2 cell lysates; Human kidney tissue; HeLa cells.
  • General notes

     

     

     This product was previously labelled as Proteasome subunit beta type 4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9397
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal macrophage proteasome (By similarity). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
    • Sequence similarities

      Belongs to the peptidase T1B family.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 26 kDa prosomal protein antibody
      • HN3 antibody
      • HsBPROS26 antibody
      • HSN3 antibody
      • Macropain beta chain antibody
      • Multicatalytic endopeptidase complex beta chain antibody
      • PROS-26 antibody
      • PROS26 antibody
      • Proteasome (prosome macropain) subunit beta type 4 antibody
      • Proteasome beta 4 subunit antibody
      • Proteasome beta chain antibody
      • Proteasome chain 3 antibody
      • Proteasome subunit beta 4 antibody
      • Proteasome subunit beta type 4 antibody
      • Proteasome subunit beta type-4 antibody
      • Proteasome subunit HsN3 antibody
      • PSB4_HUMAN antibody
      • PSMB 4 antibody
      • PSMB4 antibody
      see all

    Images

    • Immunofluorescent analysis of HeLa cells labelling PSMB4 with ab137087 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling PSMB4 with ab137087 at 1/50 dilution.

    • All lanes : Anti-PSMB4 antibody [EPR9397] (ab137087) at 1/1000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : HepG2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/200 dilution

      Predicted band size: 29 kDa

    References

    This product has been referenced in:

    • Brehm A  et al. Additive loss-of-function proteasome subunit mutations in CANDLE/PRAAS patients promote type I IFN production. J Clin Invest 125:4196-211 (2015). Read more (PubMed: 26524591) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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