Anti-PSMB5/MB1 antibody (ab167341)

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Overview

  • Product name

    Anti-PSMB5/MB1 antibody
    See all PSMB5/MB1 primary antibodies

  • Description

    Mouse polyclonal to PSMB5/MB1

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human PSMB5/MB1 aa 1-263.
    Sequence:

    MALASVLERPLPVNQRGFFGLGGRADLLDLGPGSLSDGLSLAAPGWGVPE EPGIEMLHGTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYL LGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYK GMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDR GYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDN VADLHEKYSGSTP


    Database link: P28074

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • PSMB5/MB1 transfected 293T cell line lysate. HeLa cells.

  • General notes

    Previously labelled as PSMB5. 

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage throught the Nrf2-ARE pathway.

  • Sequence similarities

    Belongs to the peptidase T1B family.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • DKFZp459C139 antibody
      • EC 3.4.25.1 antibody
      • LMPX antibody
      • Macropain epsilon chain antibody
      • MB1 antibody
      • MGC104214 antibody
      • MGC118075 antibody
      • MGC134464 antibody
      • Multicatalytic endopeptidase complex epsilon chain antibody
      • Proteasome (prosome, macropain) subunit, beta type, 5 antibody
      • Proteasome beta 5 subunit antibody
      • Proteasome catalytic subunit 3 antibody
      • Proteasome chain 6 antibody
      • Proteasome epsilon chain antibody
      • Proteasome subunit beta type-5 antibody
      • Proteasome subunit MB1 antibody
      • Proteasome subunit X antibody
      • Proteasome subunit, beta type, 5 antibody
      • Proteasome subunit, beta-5 antibody
      • PSB5_HUMAN antibody
      • PSMB5 antibody
      • PSX large multifunctional protease X antibody
      • X antibody
      see all

    Images

    • Western blot - Anti-PSMB5/MB1 antibody (ab167341)
      Western blot - Anti-PSMB5/MB1 antibody (ab167341)
      All lanes : Anti-PSMB5/MB1 antibody (ab167341) at 1 µg/ml

      Lane 1 : PSMB5/MB1 transfected 293T cell line lysate
      Lane 2 : Non-transfected 293T cell line lysate

      Lysates/proteins at 15 µl per lane.

      Predicted band size: 28 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PSMB5/MB1 antibody (ab167341)
      Immunocytochemistry/ Immunofluorescence - Anti-PSMB5/MB1 antibody (ab167341)

      Immunofluorescent analysis of HeLa cells labeling PSMB5/MB1 with ab167341 at 10 µg/ml.

    References

    ab167341 has not yet been referenced specifically in any publications.

    Publishing research using ab167341? Please let us know so that we can cite the reference in this datasheet.

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