Overview

  • Product name

  • Description

    Rabbit polyclonal to PSMC5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 66-406 of Human PSMC5

  • Positive control

    • A431, HepG2, Neuro2A, GL261, C8D30 and PC12 whole cell lysates; A549 cells; Cal27 xenograft tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
  • Sequence similarities

    Belongs to the AAA ATPase family.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 26S protease regulatory subunit 8 antibody
    • 26S proteasome AAA-ATPase subunit RPT6 antibody
    • Cim3 antibody
    • MSUG1 protein antibody
    • p45 antibody
    • p45/SUG antibody
    • Proteasome 26S ATPase subunit 5 antibody
    • Proteasome 26S subunit ATPase 5 antibody
    • Proteasome prosome macropain 26S subunit ATPase 5 antibody
    • Proteasome subunit p45 antibody
    • PRS8_HUMAN antibody
    • PSMC5 antibody
    • Rpt6 antibody
    • S8 antibody
    • SUG1 antibody
    • Tat binding protein homolog 10 antibody
    • TBP10 antibody
    • Thyroid hormone receptor interacting protein 1 antibody
    • Thyroid hormone receptor-interacting protein 1 antibody
    • Thyroid receptor interactor 1 antibody
    • TRIP1 antibody
    • TRIP1(SUG1) antibody
    see all

Images

  • All lanes : Anti-PSMC5 antibody (ab137476) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Anti-PSMC5 antibody (ab137476) at 1/1000 dilution + PC12 whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE
  • All lanes : Anti-PSMC5 antibody (ab137476) at 1/1000 dilution

    Lane 1 : Neuro2A whole cell
    lysate
    Lane 2 : GL261 whole cell
    lysate
    Lane 3 : C8D30 whole cell
    lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A549 cells labelling PSMC5 with ab137476 at 1/200 dilution. Lower image merged with DNA probe.
  • Immunohistochemical analysis of paraffin embedded Cal27 xenograft labelling PSMC5 with ab137476 at a dilution of 1/500.

References

ab137476 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137476.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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