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Rabbit polyclonal to PSMC5
Predicted to work with:
Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 3-207 of Human PSMC5 (UniProt: P62195).
A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells; Human Ca922 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used
1/100 - 1/1000.
The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
Belongs to the AAA ATPase family.
Information by UniProt
26S protease regulatory subunit 8 antibody
26S proteasome AAA-ATPase subunit RPT6 antibody
Western blot - Anti-PSMC5 antibody (ab154908)
Anti-PSMC5 antibody (ab154908) at 1/5000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 46 kDa 10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody (ab154908)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PSMC5 with ab154908 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody (ab154908)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Ca922 xenograft tissue labeling PSMC5 with ab154908 at 1/500 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"