Overview

  • Product name

    Anti-PSMC5 antibody [EPR13565(B)]
    See all PSMC5 primary antibodies
  • Description

    Rabbit monoclonal [EPR13565(B)] to PSMC5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PSMC5 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P62195

  • Positive control

    • WB: HepG2, A431, 293T Mouse heart, Mouse brain, and Rat brain lysate; IHC-P: human lung carcinoma, thyroid carcinoma, mouse liver, and rat liver tissues; ICC/IF: HeLa and PSM5C cells; FC: HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13565(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 46 kDa.

For unpurified use at 1/10000 - 1/50000.

Flow Cyt 1/90 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/500.
IHC-P 1/2000.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100.

IP 1/10 - 1/100.

Target

  • Function

    The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
  • Sequence similarities

    Belongs to the AAA ATPase family.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 26S protease regulatory subunit 8 antibody
    • 26S proteasome AAA-ATPase subunit RPT6 antibody
    • Cim3 antibody
    • MSUG1 protein antibody
    • p45 antibody
    • p45/SUG antibody
    • Proteasome 26S ATPase subunit 5 antibody
    • Proteasome 26S subunit ATPase 5 antibody
    • Proteasome prosome macropain 26S subunit ATPase 5 antibody
    • Proteasome subunit p45 antibody
    • PRS8_HUMAN antibody
    • PSMC5 antibody
    • Rpt6 antibody
    • S8 antibody
    • SUG1 antibody
    • Tat binding protein homolog 10 antibody
    • TBP10 antibody
    • Thyroid hormone receptor interacting protein 1 antibody
    • Thyroid hormone receptor-interacting protein 1 antibody
    • Thyroid receptor interactor 1 antibody
    • TRIP1 antibody
    • TRIP1(SUG1) antibody
    see all

Images

  • All lanes : Anti-PSMC5 antibody [EPR13565(B)] (ab178681) at 1/1000 dilution (Purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
    Lane 3 : Mouse heart lysates
    Lane 4 : Mouse brain lysates
    Lane 5 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • ab178681 (Purified) at 1/40 dilution (2µg) immunoprecipitating PSMC5 in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate (10µg)
    Lane 2 (+): ab178681 & HepG2 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab178681 in HepG2 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling PSMC5 with Purified ab178681 at 1/2000 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSMC5 with purified ab178681 at 1/90 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSMC5 with Purifiedab178681 at 1/500 dilution (1.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling PSMC5 with Purified ab178681 at 1/2000 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling PSMC5 with Purified ab178681 at 1/2000 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • All lanes : Anti-PSMC5 antibody [EPR13565(B)] (ab178681) at 1/10000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 46 kDa

  • Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling PSMC5 with unpurified ab178681 at 1/50 dilution.

  • Immunofluorescent analysis of HeLa cells labeling PSMC5 with unpurified ab178681 at 1/100 dilution.

  • Flow cytometric analysis of permeabilized Hela cells labeling PSMC5 with unpurified ab178681 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

  • Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1XPBS (negative control) using unpurified ab178681 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

References

This product has been referenced in:

  • Li X  et al. Inhibition of AZIN2-sv induces neovascularization and improves prognosis after myocardial infarction by blocking ubiquitin-dependent talin1 degradation and activating the Akt pathway. EBioMedicine N/A:N/A (2018). Read more (PubMed: 30545799) »
  • Sawhney S  et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One 10:e0116270 (2015). WB ; Mouse . Read more (PubMed: 25643152) »
See all 2 Publications for this product

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