Product nameAnti-PSMC5 antibody [EPR13566(B)] - C-terminal
See all PSMC5 primary antibodies
DescriptionRabbit monoclonal [EPR13566(B)] to PSMC5 - C-terminal
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PSMC5 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P62195
- HepG2, HeLa and 293T lysates; Human pancreas tissue; 293T and HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
Sequence similaritiesBelongs to the AAA ATPase family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 26S protease regulatory subunit 8 antibody
- 26S proteasome AAA-ATPase subunit RPT6 antibody
- Cim3 antibody
All lanes : Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495) at 1/10000 dilution
Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : 293T lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 46 kDa
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PSMC5 with ab180495 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow Cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling PSMC5 with ab180495 at 1/30 dilution (green) compared to a negative control Rabbit monoclonal IgG (red) follwed by Goat anti rabbit IgG (FITC) secondary at 1/75 dilution.
Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling PSMC5 with ab180495 at 1/250 dilution (red) followed by with Goat anti rabbit IgG (Dylight 555) . DAPI staining (blue).
ab180495 has not yet been referenced specifically in any publications.