Product nameAnti-PSMC6 antibody [p42-23]
See all PSMC6 primary antibodies
DescriptionMouse monoclonal [p42-23] to PSMC6
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Recombinant full length protein (Human).
- HeLa S3 cytosolic preparation and human placental proteasome preparation.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.065% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesPartially purified immunoglobulin preparation.
Our Abpromise guarantee covers the use of ab22639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/1000. PubMed: 18986984|
|WB||Use at an assay dependent concentration. Detects a band of approximately 44 kDa.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThe 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
Sequence similaritiesBelongs to the AAA ATPase family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 26S protease regulatory subunit 10B antibody
- 26S protease regulatory subunit S10B antibody
- 26S proteasome AAA-ATPase subunit RPT4 antibody
Lane 1 : Anti-PSMC6 antibody [p42-23] (ab22639) at 1/5000 dilution (Identification of 44KDa Rpt4 in HeLa S3 cytosolic preparation)
Lane 2 : Anti-PSMC6 antibody [p42-23] (ab22639) at 1/5000 dilution (Identificaition of purified protein)
Lane 3 : Anti-PSMC6 antibody [p42-23] (ab22639) at 1/5000 dilution (Identification of proteasome in human placenta )
All lanes :
Developed using the ECL technique.
Observed band size: 44 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab22639 staining PSMC6 in mock transfected rat primary neurons by Immunocytochemistry/ Immunofluorescence. Primary cultures of rat hippocampal neurons were prepared from E18 rats. Cells were fixed in 4% paraformaldehyde and incubated with primary antibody at 1/1000 dilution. The image show red staining with ab22639, nucleus was stained blue with Hoechst H33258 and right image show merged images. The green color seen in right image is the GFP staining in similar experiment.
IHC image of ab22639 staining in human normal pancrease formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22639, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Overlay histogram showing HeLa cells stained with ab22639 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22639, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Rousseau E et al. Misfolding of proteins with a polyglutamine expansion is facilitated by proteasomal chaperones. J Biol Chem 284:1917-29 (2009). WB, ICC/IF ; Human . Read more (PubMed: 18986984) »
- Hendil KB et al. Simultaneous binding of PA28 and PA700 activators to 20 S proteasomes. Biochem J 332 ( Pt 3):749-54 (1998). Read more (PubMed: 9620878) »