Key features and details
- Rabbit polyclonal to PSMD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PSMD1 antibody
See all PSMD1 primary antibodies
DescriptionRabbit polyclonal to PSMD1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan
- 293T, HeLa and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab140677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 105 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActs as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Sequence similaritiesBelongs to the proteasome subunit S1 family.
Contains 10 PC repeats.
- Information by UniProt
- MGC133041 antibody
- 26S proteasome non ATPase regulatory subunit 1 antibody
- 26S proteasome non ATPase subunit 1 antibody
All lanes : Anti-PSMD1 antibody (ab140677) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 3 minutes
Detection of PSMD1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab140677 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 3 seconds.
ab140677 has been referenced in 1 publication.
- Xiong W et al. Prognostic Significance of PSMD1 Expression in Patients with Gastric Cancer. J Cancer 10:4357-4367 (2019). PubMed: 31413756