Overview

  • Product name

  • Description

    Rabbit polyclonal to PSMD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1-50 of Human PSMD1 (NP_002798.2).

  • Positive control

    • 293T, HeLa and Jurkat whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • Sequence similarities

    Belongs to the proteasome subunit S1 family.
    Contains 10 PC repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC133041 antibody
    • 26S proteasome non ATPase regulatory subunit 1 antibody
    • 26S proteasome non ATPase subunit 1 antibody
    • 26S proteasome non-ATPase regulatory subunit 1 antibody
    • 26S proteasome regulatory subunit rpn2 antibody
    • 26S proteasome regulatory subunit S1 antibody
    • 26S proteasome subunit p112 antibody
    • MGC133040 antibody
    • P112 antibody
    • Proteasome (prosome macropain) 26S subunit, non ATPase 1 antibody
    • Psmd1 antibody
    • PSMD1_HUMAN antibody
    • RPN2 antibody
    • S1 antibody
    see all

Images

  • All lanes : Anti-PSMD1 antibody (ab140677) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 105 kDa


    Exposure time: 3 minutes
  • Detection of PSMD1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab140677 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 3 seconds.

References

ab140677 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab140677.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up