Anti-PSMD1 antibody (ab140682)
Key features and details
- Rabbit polyclonal to PSMD1
- Suitable for: IHC-Fr, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PSMD1 antibody
See all PSMD1 primary antibodies -
Description
Rabbit polyclonal to PSMD1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Non human primates, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to a region between residues 903-953 of Human PSMD1 (NP_002798.2).
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Positive control
- WB: HEK293T, HeLa, NIH3T3 and Jurkat whole cell lysate. IP: HEK293T cells. IHC-Fr: Human breast carcinoma, Mouse renal cell carcinoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/1000 - 1/5000.
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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IHC-Fr
1/1000 - 1/5000. |
WB
1/2000 - 1/10000. Predicted molecular weight: 105 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. -
Sequence similarities
Belongs to the proteasome subunit S1 family.
Contains 10 PC repeats. - Information by UniProt
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Database links
- Entrez Gene: 424926 Chicken
- Entrez Gene: 470674 Chimpanzee
- Entrez Gene: 5707 Human
- Entrez Gene: 70247 Mouse
- Entrez Gene: 100153828 Pig
- Entrez Gene: 83806 Rat
- SwissProt: Q5F418 Chicken
- SwissProt: Q99460 Human
see all -
Alternative names
- MGC133041 antibody
- 26S proteasome non ATPase regulatory subunit 1 antibody
- 26S proteasome non ATPase subunit 1 antibody
see all
Images
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Immunohistochemical analysis of frozen section of Human breast carcinoma tissue with ab140682 at 1:5,000 (0.2µg/ml) dilution.
Detection: DAB
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Immunohistochemical analysis of frozen section of Mouse renal cell carcinoma tissue with ab140682 at 1:5,000 (0.2µg/ml) dilution.
Detection: DAB
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All lanes : Anti-PSMD1 antibody (ab140682) at 0.1 µg/ml
Lane 1 : Whole cell lysate prepared from 293T cells at 50 µg
Lane 2 : Whole cell lysate prepared from 293T cells at 15 µg
Lane 3 : Whole cell lysate prepared from HeLa cells at 50 µg
Lane 4 : Whole cell lysate prepared from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 10 seconds -
Anti-PSMD1 antibody (ab140682) at 0.1 µg/ml + whole cell lysate prepared from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 seconds -
ab140682 at 1 µg/ml detecting PSMD1 in 293T whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes a different epitope of PSMD1.
Lane 2: ab140682 at 6 µg/mg of lysate.
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab140682 has been referenced in 6 publications.
- Bashore C et al. Targeted degradation via direct 26S proteasome recruitment. Nat Chem Biol 19:55-63 (2023). PubMed: 36577875
- Wang Y et al. Tsc2 knockout counteracts ubiquitin-proteasome system insufficiency and delays photoreceptor loss in retinitis pigmentosa. Proc Natl Acad Sci U S A 119:e2118479119 (2022). PubMed: 35275792
- Olshina MA et al. Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins. Antioxid Redox Signal 32:636-655 (2020). PubMed: 31903784
- Turakhiya A et al. ZFAND1 Recruits p97 and the 26S Proteasome to Promote the Clearance of Arsenite-Induced Stress Granules. Mol Cell 70:906-919.e7 (2018). PubMed: 29804830
- Lobanova ES et al. Increased proteasomal activity supports photoreceptor survival in inherited retinal degeneration. Nat Commun 9:1738 (2018). PubMed: 29712894
- Epanchintsev A et al. Cockayne's Syndrome A and B Proteins Regulate Transcription Arrest after Genotoxic Stress by Promoting ATF3 Degradation. Mol Cell 68:1054-1066.e6 (2017). PubMed: 29225035