Overview

  • Product name

  • Description

    Rabbit polyclonal to PSMD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Non human primates, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residues 903-953 of Human PSMD1 (NP_002798.2).

  • Positive control

    • 293T, HeLa, NIH3T3 and Jurkat whole cell lysate (ab7899).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-PSMD1 antibody (ab140682) at 0.1 µg/ml

    Lane 1 : Whole cell lysate prepared from 293T cells at 50 µg
    Lane 2 : Whole cell lysate prepared from 293T cells at 15 µg
    Lane 3 : Whole cell lysate prepared from HeLa cells at 50 µg
    Lane 4 : Whole cell lysate prepared from Jurkat cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 105 kDa


    Exposure time: 10 seconds
  • Anti-PSMD1 antibody (ab140682) at 0.1 µg/ml + whole cell lysate prepared from NIH3T3 cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 105 kDa


    Exposure time: 30 seconds
  • ab140682 at 1 µg/ml detecting PSMD1 in 293T whole cell lysate by WB following IP.

    Lane 1: IP with an antibody which recognizes a different epitope of PSMD1.
    Lane 2: ab140682 at 6 µg/mg of lysate.
    Lane 3: Control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 3 seconds

References

This product has been referenced in:

  • Lobanova ES  et al. Increased proteasomal activity supports photoreceptor survival in inherited retinal degeneration. Nat Commun 9:1738 (2018). Read more (PubMed: 29712894) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - whole cell (Egg extract (total and chromatin))
Specification
Egg extract (total and chromatin)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

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Verified customer

Submitted Jan 20 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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