For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    psmd1-antibody-ab2941.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Amino Acids
Share by email

Anti-PSMD1 antibody (ab2941)

  • Datasheet
Submit a review Q&A (3)References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-PSMD1 antibody (ab2941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD1 antibody (ab2941)
  • Immunocytochemistry/ Immunofluorescence - Anti-PSMD1 antibody (ab2941)

Key features and details

  • Rabbit polyclonal to PSMD1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-PSMD1 antibody
    See all PSMD1 primary antibodies
  • Description

    Rabbit polyclonal to PSMD1
  • Host species

    Rabbit
  • Specificity

    Detects proteasome Rpn2 from human cells.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human PSMD1 aa 929-953.
    Sequence:

    AAHGPKIEEEEQEPEPPEPFEYIDD

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Rat
Chicken
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).

By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract.

IHC-P
Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 1 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).

By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract.

IHC-P
Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • Sequence similarities

    Belongs to the proteasome subunit S1 family.
    Contains 10 PC repeats.
  • Target information above from: UniProt accession Q99460 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 424926 Chicken
    • Entrez Gene: 5707 Human
    • Entrez Gene: 83806 Rat
    • SwissProt: Q5F418 Chicken
    • SwissProt: Q99460 Human
    • SwissProt: O88761 Rat
    • Unigene: 3887 Human
    • Unigene: 2891 Rat
    • Alternative names

      • MGC133041 antibody
      • 26S proteasome non ATPase regulatory subunit 1 antibody
      • 26S proteasome non ATPase subunit 1 antibody
      • 26S proteasome non-ATPase regulatory subunit 1 antibody
      • 26S proteasome regulatory subunit rpn2 antibody
      • 26S proteasome regulatory subunit S1 antibody
      • 26S proteasome subunit p112 antibody
      • MGC133040 antibody
      • P112 antibody
      • Proteasome (prosome macropain) 26S subunit, non ATPase 1 antibody
      • Psmd1 antibody
      • PSMD1_HUMAN antibody
      • RPN2 antibody
      • S1 antibody
      see all

    Images

    • Western blot - Anti-PSMD1 antibody (ab2941)
      Western blot - Anti-PSMD1 antibody (ab2941)

      Western blot of proteasome Rpn2 from HeLa cell extract using ab2941.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD1 antibody (ab2941)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD1 antibody (ab2941)
      ab2941 (4µg/ml) staining Rpn2 in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclei and cytoplasm within the sebaceous gland.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
    • Immunocytochemistry/ Immunofluorescence - Anti-PSMD1 antibody (ab2941)
      Immunocytochemistry/ Immunofluorescence - Anti-PSMD1 antibody (ab2941)
      ICC/IF image of ab2941 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2941, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab2941? Please let us know so that we can cite the reference in this datasheet.

    ab2941 has been referenced in 3 publications.

    • Bhat K  et al. Serum erythropoietin levels, breast cancer and breast cancer-initiating cells. Breast Cancer Res 21:17 (2019). PubMed: 30700319
    • Lu X  et al. Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets. Nat Commun 8:15540 (2017). PubMed: 28598414
    • Moscovitz O  et al. The Parkinson's-associated protein DJ-1 regulates the 20S proteasome. Nat Commun 6:6609 (2015). WB . PubMed: 25833141

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    What is the concentration? Abcam lot# 119084

    Read More

    Abcam community

    Verified customer

    Asked on Sep 09 2005

    Answer

    Thank you for your phone call. The concentration is 1 mg/ml. If you have any additional questions, please let us know. Have a great weekend!

    Read More

    Abcam Scientific Support

    Answered on Sep 09 2005

    Question

    I was wondering if this antibody has been successfully tested in immunoprecipitation assays. Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 25 2005

    Answer

    Thanks for your enquiry. To our knowledge, this antibody has yet to be tested in IP. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please let us know.

    Read More

    Abcam Scientific Support

    Answered on Jan 25 2005

    Question

    I would like to know if the proteasome 19S antibody ab2941 could work with rat brain slices, because we would like to study the proteasome expression by immunohistochemistry. you claim it works with human and cow tissues.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 04 2003

    Answer

    All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with rat has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Dec 04 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.