Anti-PSMD1 antibody (ab2941)

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Overview

  • Product name

    Anti-PSMD1 antibody
    See all PSMD1 primary antibodies

  • Description

    Rabbit polyclonal to PSMD1

  • Host species

    Rabbit

  • Specificity

    Detects proteasome Rpn2 from human cells.

  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Cow, Human, Non human primates
    Predicted to work with: Rat, Chicken

  • Immunogen

    Synthetic peptide corresponding to Human PSMD1 aa 929-953.
    Sequence:

    AAHGPKIEEEEQEPEPPEPFEYIDD

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab2941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).

By Western blot, this antibody detects an ~105 kDa protein representing proteasome 19S subunit S1 from HeLa cell extract.
IHC-Fr Use a concentration of 15 µg/ml.
IHC-P Use a concentration of 15 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

Target

Images

  • Western blot - Anti-PSMD1 antibody (ab2941)
    Western blot - Anti-PSMD1 antibody (ab2941)

    Western blot of proteasome Rpn2 from HeLa cell extract using ab2941.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD1 antibody (ab2941)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD1 antibody (ab2941)
    ab2941 (4µg/ml) staining Rpn2 in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclei and cytoplasm within the sebaceous gland.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunocytochemistry/ Immunofluorescence - Anti-PSMD1 antibody (ab2941)
    Immunocytochemistry/ Immunofluorescence - Anti-PSMD1 antibody (ab2941)
    ICC/IF image of ab2941 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2941, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Bhat K  et al. Serum erythropoietin levels, breast cancer and breast cancer-initiating cells. Breast Cancer Res 21:17 (2019). Read more (PubMed: 30700319) »
  • Lu X  et al. Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets. Nat Commun 8:15540 (2017). Read more (PubMed: 28598414) »
See all 3 Publications for this product

Publishing research using ab2941? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

What is the concentration? Abcam lot# 119084

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Answer

Thank you for your phone call. The concentration is 1 mg/ml. If you have any additional questions, please let us know. Have a great weekend!

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Question

I was wondering if this antibody has been successfully tested in immunoprecipitation assays. Thanks.

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Answer

Thanks for your enquiry. To our knowledge, this antibody has yet to be tested in IP. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please let us know.

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Question

I would like to know if the proteasome 19S antibody ab2941 could work with rat brain slices, because we would like to study the proteasome expression by immunohistochemistry. you claim it works with human and cow tissues.

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Answer

All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with rat has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any more questions, please contact us again.

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