Anti-PSMD11 antibody (ab221645)
Key features and details
- Rabbit polyclonal to PSMD11
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PSMD11 antibody
See all PSMD11 primary antibodies -
Description
Rabbit polyclonal to PSMD11 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human PSMD11 aa 1-100.
Database link: O00231 -
Positive control
- ICC/IF: U-2 OS cells. WB: RT4 cell lysate. IHC-P: Human testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 47 kDa.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 47 kDa. |
Target
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Function
Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity. -
Tissue specificity
Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate. -
Sequence similarities
Belongs to the proteasome subunit S9 family.
Contains 1 PCI domain. -
Post-translational
modificationsPhosphorylated by AMPK. -
Cellular localization
Nucleus. Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 513461 Cow
- Entrez Gene: 5717 Human
- Entrez Gene: 69077 Mouse
- Entrez Gene: 303353 Rat
- Omim: 604449 Human
- SwissProt: Q2KI42 Cow
- SwissProt: O00231 Human
- SwissProt: Q8BG32 Mouse
see all -
Alternative names
- 26S proteasome non-ATPase regulatory subunit 11 antibody
- 26S proteasome regulatory subunit 9 antibody
- 26S proteasome regulatory subunit p44.5 antibody
see all
Images
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Anti-PSMD11 antibody (ab221645) at 1/100 dilution + RT4 (human urinary bladder cancer cell line) cell lysate.
Predicted band size: 47 kDa -
Paraffin-embedded human testis tissue stained for PSMD11 using ab221645 at 1/50 dilution in Immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PSMD11 (green) using ab221645 at 4 µg/ml in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab221645 has not yet been referenced specifically in any publications.