Key features and details
- Rabbit polyclonal to PSMD11
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PSMD11 antibody
See all PSMD11 primary antibodies
DescriptionRabbit polyclonal to PSMD11
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- ICC/IF: U-2 OS cells. WB: RT4 cell lysate. IHC-P: Human testis tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 47 kDa.|
FunctionComponent of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Tissue specificityHighly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.
Sequence similaritiesBelongs to the proteasome subunit S9 family.
Contains 1 PCI domain.
modificationsPhosphorylated by AMPK.
Cellular localizationNucleus. Cytoplasm > cytosol.
- Information by UniProt
- 26S proteasome non-ATPase regulatory subunit 11 antibody
- 26S proteasome regulatory subunit 9 antibody
- 26S proteasome regulatory subunit p44.5 antibody
Anti-PSMD11 antibody (ab221645) at 1/100 dilution + RT4 (human urinary bladder cancer cell line) cell lysate.
Predicted band size: 47 kDa
Paraffin-embedded human testis tissue stained for PSMD11 using ab221645 at 1/50 dilution in Immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PSMD11 (green) using ab221645 at 4 µg/ml in ICC/IF.
ab221645 has not yet been referenced specifically in any publications.