Overview

  • Product name

  • Description

    Rabbit polyclonal to PSMD11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human PSMD11 aa 4-72.
    Sequence:

    AAVVEFQRAQSLLSTDREASIDILHSIVKRDIQENDEEAVQVKEQSILEL GSLLAKTGQAAELGGLLKY


    Database link: O00231

  • Positive control

    • ICC/IF: U-2 OS cells. WB: RT4 cell lysate. IHC-P: Human testis tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab221645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

WB 1/100 - 1/250. Predicted molecular weight: 47 kDa.

Target

  • Function

    Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
  • Tissue specificity

    Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.
  • Sequence similarities

    Belongs to the proteasome subunit S9 family.
    Contains 1 PCI domain.
  • Post-translational
    modifications

    Phosphorylated by AMPK.
  • Cellular localization

    Nucleus. Cytoplasm > cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • 26S proteasome non-ATPase regulatory subunit 11 antibody
    • 26S proteasome regulatory subunit 9 antibody
    • 26S proteasome regulatory subunit p44.5 antibody
    • 26S proteasome regulatory subunit RPN6 antibody
    • 26S proteasome regulatory subunit S9 antibody
    • MGC3844 antibody
    • p44.5 antibody
    • protease 26S, subunit, 9 antibody
    • proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 antibody
    • proteasome 26S subunit, non-ATPase, 11 antibody
    • PSD11_HUMAN antibody
    • PSMD 11 antibody
    • PSMD11 antibody
    • Rpn6 antibody
    • S9 antibody
    see all

Images

  • Anti-PSMD11 antibody (ab221645) at 1/100 dilution + RT4 (human urinary bladder cancer cell line) cell lysate.

    Predicted band size: 47 kDa

  • Paraffin-embedded human testis tissue stained for PSMD11 using ab221645 at 1/50 dilution in Immunohistochemical analysis.

  • PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PSMD11 (green) using ab221645 at 4 µg/ml in ICC/IF. 

References

ab221645 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221645.
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