Key features and details
- Rabbit polyclonal to PSMD11
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PSMD11 antibody
See all PSMD11 primary antibodies
DescriptionRabbit polyclonal to PSMD11
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide, corresponding to a region within amino acids 372-422 of Human PSMD11 ((NP_002806.2).
- Human HeLa and 293T cell lines. Mouse NIH3T3 cell line.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 47 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionComponent of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Tissue specificityHighly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate.
Sequence similaritiesBelongs to the proteasome subunit S9 family.
Contains 1 PCI domain.
modificationsPhosphorylated by AMPK.
Cellular localizationNucleus. Cytoplasm > cytosol.
- Information by UniProt
- 26S proteasome non-ATPase regulatory subunit 11 antibody
- 26S proteasome regulatory subunit 9 antibody
- 26S proteasome regulatory subunit p44.5 antibody
All lanes : Anti-PSMD11 antibody (ab99414) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 3 minutes
Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99414 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane
Western Blot: PSMD11 antibody (ab99414) at 0.4 µg/ml
developed using the ECL technique
Exposure time : 30 seconds
Predicted band size : 47 kDa
ab99414 has not yet been referenced specifically in any publications.