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    psmd12-antibody-ab137932.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome
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Anti-PSMD12 antibody (ab137932)

  • Datasheet
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Western blot - Anti-PSMD12 antibody (ab137932)

    Key features and details

    • Rabbit polyclonal to PSMD12
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-PSMD12 antibody
      See all PSMD12 primary antibodies
    • Description

      Rabbit polyclonal to PSMD12
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human PSMD12 (internal sequence).

    • Positive control

      • HT29 cell lysate
    • General notes

       This product was previously labelled as 26S proteasome non-ATPase regulatory subunit 12

       

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

      PBS is without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Proteasome

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HT-29 whole cell lysate (ab3952)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab137932 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Mouse
    Rat
    Application Abreviews Notes
    WB (2)
    1/500 - 1/1000. Predicted molecular weight: 52 kDa.
    Notes
    WB
    1/500 - 1/1000. Predicted molecular weight: 52 kDa.

    Target

    • Function

      Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
    • Sequence similarities

      Belongs to the proteasome subunit p55 family.
      Contains 1 PCI domain.
    • Target information above from: UniProt accession O00232 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5718 Human
      • Entrez Gene: 66997 Mouse
      • Entrez Gene: 287772 Rat
      • Omim: 604450 Human
      • SwissProt: O00232 Human
      • SwissProt: Q9D8W5 Mouse
      • Unigene: 592689 Human
      • Unigene: 21667 Mouse
      • Unigene: 15873 Rat
      see all
    • Alternative names

      • 26S proteasome non-ATPase regulatory subunit 12 antibody
      • 26S proteasome regulatory subunit p55 antibody
      • 26S proteasome regulatory subunit RPN5 antibody
      • MGC75406 antibody
      • p55 antibody
      • proteasome (prosome, macropain) 26S subunit, non ATPase12 antibody
      • Proteasome 26S non ATPase subunit 12 isoform 2 antibody
      • PSD12_HUMAN antibody
      • PSMD12 antibody
      • Rpn5 antibody
      see all

    Images

    • Western blot - Anti-PSMD12 antibody (ab137932)
      Western blot - Anti-PSMD12 antibody (ab137932)
      Anti-PSMD12 antibody (ab137932) at 1/500 dilution + HT29 cell lysate at 30 µg

      Predicted band size: 52 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab137932? Please let us know so that we can cite the reference in this datasheet.

    ab137932 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-PSMD12 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (Neuronal tissue)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Treatment
    RIPA buffer with protease inhibitor
    Specification
    Neuronal tissue
    Blocking step
    Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 24 2016

    Western blot abreview for Anti-26S Proteasome regulatory subunit p55 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Rat Tissue lysate - whole (brain)
    Specification
    brain
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Grace Zhou

    Verified customer

    Submitted Jun 26 2014

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