Western blot abreview for Anti-26S Proteasome regulatory subunit p55 antibody

Average
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Rat Tissue lysate - whole (brain)
Specification
brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Diluent: TBS
Dilution
1/800

Secondary antibody

Name
Non-Abcam antibody was used: ANTI RABBIT IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Bands
Specific: 50 kDa Non-specific: 75, 80, 120 kDa
Exposure
2 minute(s) and 0 second(s)
Positive control
NO POSITIVE CONTROL

Additional data

Abcam response
We appreciate the valuable data provided in this Abreview. The detection of the rat 26S Proteasome regulatory subunit p55 is predicted due to a high alignment of the immunogen with the rat amino acid sequence of the target protein. The expected band could be obtained in this case, however some additional nonspecific bands are also detected. We would welcome other researcher’s feedback using this antibody with rat samples.

Dr. Grace Zhou

Verified customer

Submitted Jun 26 2014

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