Western blot abreview for Anti-PSMD12 antibody

Poor
Abreviews
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Neuronal tissue)
Gel Running Conditions
Reduced Denaturing (7.5%)
Treatment
RIPA buffer with protease inhibitor
Specification
Neuronal tissue
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk Block
Dilution
1/100

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: AP-conjugated AffiniPure goat anti-rabbit igG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase

Detection

Detection method
AP
Exposure
45 minute(s) and 0 second(s)

Additional data

Additional Notes
Lane 1: 4 eyes Lane 1a: 3 brains

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Submitted May 24 2016

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