Key features and details
- Rabbit polyclonal to PSMD12
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PSMD12 antibody
See all PSMD12 primary antibodies
DescriptionRabbit polyclonal to PSMD12
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Orangutan
Recombinant fragment corresponding to Human PSMD12 aa 197-456.
KDYIRTQIISKKINTKFFQEENTEKLKLKYYNLMIQLDQHEGSYLSICKH YRAIYDTPCIQAESEKWQQALKSVVLYVILAPFDNEQSDLVHRISGDKKL EEIPKYKDLLKLFTTMELMRWSTLVEDYGMELRKGSLESPATDVFGSTEE GEKRWKDLKNRVVEHNIRIMAKYYTRITMKRMAQLLDLSVDESEAFLSNL VVNKTIFAKVDRLAGIINFQRPKDPNNLLNDWSQKLNSLMSLVNKTTHLI AKEEMIHNLQ
Database link: O00232-1
- WB: U87 and HeLa whole cell lysates. IHC-P: Human gastric cancer tissue.
This product was previously labelled as 26S proteasome non-ATPase regulatory subunit 12
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53, 51 kDa).|
FunctionActs as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Sequence similaritiesBelongs to the proteasome subunit p55 family.
Contains 1 PCI domain.
- Information by UniProt
- 26S proteasome non-ATPase regulatory subunit 12 antibody
- 26S proteasome regulatory subunit p55 antibody
- 26S proteasome regulatory subunit RPN5 antibody
All lanes : Anti-PSMD12 antibody (ab229930) at 1/1000 dilution
Lane 1 : U87 whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 53, 51 kDa
Paraffin-embedded human gastric cancer tissue stained for PSMD12 with ab229930 at a 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229930 has not yet been referenced specifically in any publications.