Product nameAnti-PSMD12 antibody
See all PSMD12 primary antibodies
DescriptionRabbit polyclonal to PSMD12
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human testis tissue. WB: RT4 and U-251 MG cell lysate; Human liver tissue lysate. ICC/IF: U-251 MG cells.
This product was previously labelled as 26S proteasome non-ATPase regulatory subunit 12
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.4 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
FunctionActs as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Sequence similaritiesBelongs to the proteasome subunit p55 family.
Contains 1 PCI domain.
- Information by UniProt
- 26S proteasome non-ATPase regulatory subunit 12 antibody
- 26S proteasome regulatory subunit p55 antibody
- 26S proteasome regulatory subunit RPN5 antibody
PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling PSMD12 using ab246940 at 4 µg/ml (green) in ICC/IF.
Paraffin-embedded human testis tissue stained for PSMD12 ab246940 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human salivary gland tissue stained for PSMD12 using ab246940 at 1/200 dilution in immunohistochemical analysis. Low expression as expected.
All lanes : Anti-PSMD12 antibody (ab246940) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
ab246940 has not yet been referenced specifically in any publications.