Key features and details
- Rabbit polyclonal to PSMD12
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PSMD12 antibody
See all PSMD12 primary antibodies
DescriptionRabbit polyclonal to PSMD12
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human testis tissue. WB: RT4 and U-251 MG cell lysate; Human liver tissue lysate. ICC/IF: U-251 MG cells.
This product was previously labelled as 26S proteasome non-ATPase regulatory subunit 12
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionActs as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Sequence similaritiesBelongs to the proteasome subunit p55 family.
Contains 1 PCI domain.
- Information by UniProt
- 26S proteasome non-ATPase regulatory subunit 12 antibody
- 26S proteasome regulatory subunit p55 antibody
- 26S proteasome regulatory subunit RPN5 antibody
PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling PSMD12 using ab246940 at 4 µg/ml (green) in ICC/IF.
Paraffin-embedded human testis tissue stained for PSMD12 ab246940 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human salivary gland tissue stained for PSMD12 using ab246940 at 1/200 dilution in immunohistochemical analysis. Low expression as expected.
All lanes : Anti-PSMD12 antibody (ab246940) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246940 has not yet been referenced specifically in any publications.