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  1. Link

    psmd13-antibody-ab229812.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome
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Anti-PSMD13 antibody (ab229812)

  • Datasheet
Submit a review Submit a question References (1)

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  • Western blot - Anti-PSMD13 antibody (ab229812)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody (ab229812)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody (ab229812)

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  • Datasheet
  • References (1)
  • Protocols

Overview

  • Product name

    Anti-PSMD13 antibody
    See all PSMD13 primary antibodies
  • Description

    Rabbit polyclonal to PSMD13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human PSMD13 aa 1-250.
    Database link: Q9UNM6

  • Positive control

    • WB: Mouse lung lysate; HEK-293T, HeLa, HepG2 and Jurkat whole cell lysates. IHC-P: Human lung cancer and adrenal gland tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HepG2 whole cell lysate (ab166833)
    • Mouse lung normal tissue lysate - total protein (ab29297)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
    • HepG2 whole cell lysate (ab7900)
    • 293T whole cell lysate (ab95494)
  • Recombinant Protein

    • Recombinant Human PSMD13 protein (ab159272)
  • Related Products

    • Recombinant Human PSMD13 protein (ab159272)
    • Recombinant Human PSMD13 protein (denatured) (ab181912)

Applications

Our Abpromise guarantee covers the use of ab229812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
  • Sequence similarities

    Belongs to the proteasome subunit S11 family.
    Contains 1 PCI domain.
  • Target information above from: UniProt accession Q9UNM6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5719 Human
    • Entrez Gene: 23997 Mouse
    • Omim: 603481 Human
    • SwissProt: Q9UNM6 Human
    • SwissProt: Q9WVJ2 Mouse
    • Unigene: 134688 Human
    • Unigene: 29760 Mouse
    • Alternative names

      • 26S proteasome non ATPase regulatory subunit 13 antibody
      • 26S proteasome non-ATPase regulatory subunit 13 antibody
      • 26S proteasome regulatory subunit p40.5 antibody
      • 26S proteasome regulatory subunit RPN9 antibody
      • 26S proteasome regulatory subunit S11 antibody
      • 26S proteasome subunit p40.5 antibody
      • HSPC027 antibody
      • P40.5 antibody
      • Proteasome (prosome, macropain) 26S subunit, non ATPase, 13 antibody
      • Proteasome 26S subunit, non-ATPase 13 antibody
      • PSD13_HUMAN antibody
      • psmD13 antibody
      • Rpn9 antibody
      • S11 antibody
      see all

    Images

    • Western blot - Anti-PSMD13 antibody (ab229812)
      Western blot - Anti-PSMD13 antibody (ab229812)
      All lanes : Anti-PSMD13 antibody (ab229812) at 1/1000 dilution

      Lane 1 : Mouse lung lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

      Secondary
      All lanes : Goat anti-rabbit IgG at 1/10000 dilution

      Predicted band size: 43 kDa
      Observed band size: 43 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody (ab229812)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody (ab229812)

      Paraffin-embedded human lung cancer tissue stained for PSMD13 using ab229812 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody (ab229812)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody (ab229812)

      Paraffin-embedded human adrenal gland tissue stained for PSMD13 using ab229812 at 1/100 dilution in immunohistochemical analysis.

    Datasheets and documents

    • Datasheet
  • References

    This product has been referenced in:

    • Wegmann S  et al. Experimental evidence for the age dependence of tau protein spread in the brain. Sci Adv 5:eaaw6404 (2019). Read more (PubMed: 31249873) »
    See 1 Publication for this product

    Publishing research using ab229812? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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