Anti-PSMD13 antibody (ab229812)
Key features and details
- Rabbit polyclonal to PSMD13
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PSMD13 antibody
See all PSMD13 primary antibodies -
Description
Rabbit polyclonal to PSMD13 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human PSMD13 aa 1-250.
Database link: Q9UNM6 -
Positive control
- WB: Mouse lung lysate; HEK-293T, HeLa, HepG2 and Jurkat whole cell lysates. IHC-P: Human lung cancer and adrenal gland tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab229812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/20 - 1/200. | |
| WB | 1/1000 - 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. -
Sequence similarities
Belongs to the proteasome subunit S11 family.
Contains 1 PCI domain. - Information by UniProt
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Database links
- Entrez Gene: 5719 Human
- Entrez Gene: 23997 Mouse
- Omim: 603481 Human
- SwissProt: Q9UNM6 Human
- SwissProt: Q9WVJ2 Mouse
- Unigene: 134688 Human
- Unigene: 29760 Mouse
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Alternative names
- 26S proteasome non ATPase regulatory subunit 13 antibody
- 26S proteasome non-ATPase regulatory subunit 13 antibody
- 26S proteasome regulatory subunit p40.5 antibody
see all
Images
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Western blot - Anti-PSMD13 antibody (ab229812)All lanes : Anti-PSMD13 antibody (ab229812) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Secondary
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody (ab229812)Paraffin-embedded human lung cancer tissue stained for PSMD13 using ab229812 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody (ab229812)Paraffin-embedded human adrenal gland tissue stained for PSMD13 using ab229812 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (1)
ab229812 has been referenced in 1 publication.
- Wegmann S et al. Experimental evidence for the age dependence of tau protein spread in the brain. Sci Adv 5:eaaw6404 (2019). PubMed: 31249873
