Recombinant Anti-PSMD13 antibody [EPR8525] (ab131054)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8525] to PSMD13
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PSMD13 antibody [EPR8525]
See all PSMD13 primary antibodies -
Description
Rabbit monoclonal [EPR8525] to PSMD13 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Y79, HeLa, HepG2, and Jurkat cell lysates; Human kidney tissue; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8525 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. -
Sequence similarities
Belongs to the proteasome subunit S11 family.
Contains 1 PCI domain. - Information by UniProt
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Database links
- Entrez Gene: 5719 Human
- Entrez Gene: 23997 Mouse
- Entrez Gene: 365388 Rat
- Omim: 603481 Human
- SwissProt: Q9UNM6 Human
- SwissProt: Q9WVJ2 Mouse
- SwissProt: B0BN93 Rat
- Unigene: 134688 Human
see all -
Alternative names
- 26S proteasome non ATPase regulatory subunit 13 antibody
- 26S proteasome non-ATPase regulatory subunit 13 antibody
- 26S proteasome regulatory subunit p40.5 antibody
see all
Images
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All lanes : Anti-PSMD13 antibody [EPR8525] (ab131054) at 1/1000 dilution
Lane 1 : Y79 (Human retinoblastoma cell line) cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
Actual band size : 43 kDa -
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling PSMD13 with ab131054 antibody at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunocytochemical analysis of HepG2 cells labelling PSMD13 with ab131054 antibody at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131054 has not yet been referenced specifically in any publications.