Recombinant
RabMAb

Recombinant Anti-PSMD13 antibody [EPR8525] (ab131054)

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Overview

  • Product name

    Anti-PSMD13 antibody [EPR8525]
    See all PSMD13 primary antibodies

  • Description

    Rabbit monoclonal [EPR8525] to PSMD13

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human PSMD13 (UniProt ID: Q9UNM6).

  • Positive control

    • Y79, HeLa, HepG2, and Jurkat cell lysates; Human kidney tissue; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8525

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

    • Sequence similarities

      Belongs to the proteasome subunit S11 family.
      Contains 1 PCI domain.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 26S proteasome non ATPase regulatory subunit 13 antibody
      • 26S proteasome non-ATPase regulatory subunit 13 antibody
      • 26S proteasome regulatory subunit p40.5 antibody
      • 26S proteasome regulatory subunit RPN9 antibody
      • 26S proteasome regulatory subunit S11 antibody
      • 26S proteasome subunit p40.5 antibody
      • HSPC027 antibody
      • P40.5 antibody
      • Proteasome (prosome, macropain) 26S subunit, non ATPase, 13 antibody
      • Proteasome 26S subunit, non-ATPase 13 antibody
      • PSD13_HUMAN antibody
      • psmD13 antibody
      • Rpn9 antibody
      • S11 antibody
      see all

    Images

    • Western blot - Anti-PSMD13 antibody [EPR8525] (ab131054)
      Western blot - Anti-PSMD13 antibody [EPR8525] (ab131054)
      All lanes : Anti-PSMD13 antibody [EPR8525] (ab131054) at 1/1000 dilution

      Lane 1 : Y79 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 43 kDa



      Actual band size : 43 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody [EPR8525] (ab131054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody [EPR8525] (ab131054)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling PSMD13 with ab131054 antibody at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-PSMD13 antibody [EPR8525] (ab131054)
      Immunocytochemistry/ Immunofluorescence - Anti-PSMD13 antibody [EPR8525] (ab131054)
      Immunocytochemical analysis of HepG2 cells labelling PSMD13 with ab131054 antibody at 1/100 dilution.

    References

    ab131054 has not yet been referenced specifically in any publications.

    Publishing research using ab131054? Please let us know so that we can cite the reference in this datasheet.

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