RecombinantRabMAb

Recombinant Anti-PSMD13 antibody [EPR8525] (ab131054)

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Western blot - Anti-PSMD13 antibody [EPR8525] (ab131054)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody [EPR8525] (ab131054)
  • Immunocytochemistry/ Immunofluorescence - Anti-PSMD13 antibody [EPR8525] (ab131054)
  • Anti-PSMD13 antibody [EPR8525] (ab131054)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8525] to PSMD13
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-PSMD13 antibody [EPR8525]
    See all PSMD13 primary antibodies

  • Description

    Rabbit monoclonal [EPR8525] to PSMD13

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human PSMD13 (UniProt ID: Q9UNM6).

  • Positive control

    • Y79, HeLa, HepG2, and Jurkat cell lysates; Human kidney tissue; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8525

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

    • Sequence similarities

      Belongs to the proteasome subunit S11 family.
      Contains 1 PCI domain.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 26S proteasome non ATPase regulatory subunit 13 antibody
      • 26S proteasome non-ATPase regulatory subunit 13 antibody
      • 26S proteasome regulatory subunit p40.5 antibody
      • 26S proteasome regulatory subunit RPN9 antibody
      • 26S proteasome regulatory subunit S11 antibody
      • 26S proteasome subunit p40.5 antibody
      • HSPC027 antibody
      • P40.5 antibody
      • Proteasome (prosome, macropain) 26S subunit, non ATPase, 13 antibody
      • Proteasome 26S subunit, non-ATPase 13 antibody
      • PSD13_HUMAN antibody
      • psmD13 antibody
      • Rpn9 antibody
      • S11 antibody
      see all

    Images

    • Western blot - Anti-PSMD13 antibody [EPR8525] (ab131054)
      Western blot - Anti-PSMD13 antibody [EPR8525] (ab131054)
      All lanes : Anti-PSMD13 antibody [EPR8525] (ab131054) at 1/1000 dilution

      Lane 1 : Y79 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 43 kDa



      Actual band size : 43 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody [EPR8525] (ab131054)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD13 antibody [EPR8525] (ab131054)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling PSMD13 with ab131054 antibody at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-PSMD13 antibody [EPR8525] (ab131054)
      Immunocytochemistry/ Immunofluorescence - Anti-PSMD13 antibody [EPR8525] (ab131054)
      Immunocytochemical analysis of HepG2 cells labelling PSMD13 with ab131054 antibody at 1/100 dilution.
    • Anti-PSMD13 antibody [EPR8525] (ab131054)
      Anti-PSMD13 antibody [EPR8525] (ab131054)

    References (0)

    Publishing research using ab131054? Please let us know so that we can cite the reference in this datasheet.

    ab131054 has not yet been referenced specifically in any publications.

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