Overview

  • Product name

  • Description

    Rabbit polyclonal to PSMD14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Gorilla, Chinese hamster, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 260-310 of Human PSMD14 (NP_005796.1).

  • Positive control

    • 293T, HeLa, Jurkat, NIH3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab140496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 35 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
  • Tissue specificity

    Widely expressed. Highest levels in heart and skeletal muscle.
  • Sequence similarities

    Belongs to the peptidase M67A family. PSMD14 subfamily.
    Contains 1 MPN (JAB/Mov34) domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 26S proteasome non-ATPase regulatory subunit 14 antibody
    • 26S proteasome regulatory subunit rpn11 antibody
    • 26S proteasome-associated PAD1 homolog 1 antibody
    • 26S proteasome-associated PAD1 homolog antibody
    • PAD1 antibody
    • PAD1, yeast, homolog of antibody
    • POH1 antibody
    • Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody
    • Proteasome 26S subunit non ATPase 14 antibody
    • PSDE_HUMAN antibody
    • Psmd14 antibody
    • RPN11 antibody
    • Testis tissue sperm binding protein Li 69n antibody
    see all

Images

  • All lanes : Anti-PSMD14 antibody (ab140496) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 35 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation of PSMD14 from 293T whole cell lysate by ab140496 at 6 µg/mg lysate (Lane 1). 1 mg for IP; 20% of IP loaded. Lane 2: IgG control.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab140496 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab140496.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up