Key features and details
- Rabbit polyclonal to PSMD14
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PSMD14 antibody
See all PSMD14 primary antibodies
DescriptionRabbit polyclonal to PSMD14
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Gorilla, Chinese hamster, Xenopus tropicalis
- 293T, HeLa, Jurkat, NIH3T3 whole cell lysates
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab140496 was affinity purified using an epitope specific to PSMD14 immobilized on solid support.
Our Abpromise guarantee covers the use of ab140496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 35 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMetalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
Tissue specificityWidely expressed. Highest levels in heart and skeletal muscle.
Sequence similaritiesBelongs to the peptidase M67A family. PSMD14 subfamily.
Contains 1 MPN (JAB/Mov34) domain.
- Information by UniProt
- 26S proteasome non-ATPase regulatory subunit 14 antibody
- 26S proteasome regulatory subunit rpn11 antibody
- 26S proteasome-associated PAD1 homolog 1 antibody
All lanes : Anti-PSMD14 antibody (ab140496) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 30 seconds
Immunoprecipitation of PSMD14 from 293T whole cell lysate by ab140496 at 6 µg/mg lysate (Lane 1). 1 mg for IP; 20% of IP loaded. Lane 2: IgG control.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab140496 has not yet been referenced specifically in any publications.