Product nameAnti-PSMD14 antibody
See all PSMD14 primary antibodies
DescriptionRabbit polyclonal to PSMD14
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human PSMD14 aa 216-310. The exact sequence is proprietary. (With tag).
Database link: O00487
- 293T, SKOV3, 231 and mouse NIH 3T3 cell lysates; Human liver cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Note: PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab182762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/100.|
|WB||1/500 - 1/2000. Predicted molecular weight: 35 kDa.|
FunctionMetalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
Tissue specificityWidely expressed. Highest levels in heart and skeletal muscle.
Sequence similaritiesBelongs to the peptidase M67A family. PSMD14 subfamily.
Contains 1 MPN (JAB/Mov34) domain.
- Information by UniProt
- 26S proteasome non-ATPase regulatory subunit 14 antibody
- 26S proteasome regulatory subunit rpn11 antibody
- 26S proteasome-associated PAD1 homolog 1 antibody
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling PSMD14 with ab182762 at 1/20 dilution (left panel). Right panel is treated with the immunogen.
All lanes : Anti-PSMD14 antibody (ab182762) at 1/200 dilution
Lane 1 : 293T cell lysate
Lane 2 : SKOV3 cell lysate
Lane 3 : NIH 3T3 cell lysate
Lane 4 : 231 cell lysate
Lysates/proteins at 50 µg per lane.
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 1 minute
Gel: 10% SDS-PAGE
ab182762 has not yet been referenced specifically in any publications.