Recombinant Anti-PSMD14 antibody [EPR4257] (ab109123)
![Western blot - Anti-PSMD14 antibody [EPR4257] (ab109123)](https://www.abcam.com/ps/products/109/ab109123/Images/ab109123-121715-anti-psmd14-antibody-epr4257-western-blot.jpg "Western blot - Anti-PSMD14 antibody [EPR4257] (ab109123)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4257] to PSMD14
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PSMD14 antibody [EPR4257]
See all PSMD14 primary antibodies -
Description
Rabbit monoclonal [EPR4257] to PSMD14 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PSMD14 aa 150-250. The exact sequence is proprietary.
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Positive control
- HepG2, A431, HeLa, Namalwa, K562 and MCF7 cell lysates; Human colon tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4257 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab109123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 35 kDa. | |
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min). | |
| ICC/IF | 1/500 - 1/1000. |
Target
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Function
Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear. -
Tissue specificity
Widely expressed. Highest levels in heart and skeletal muscle. -
Sequence similarities
Belongs to the peptidase M67A family. PSMD14 subfamily.
Contains 1 MPN (JAB/Mov34) domain. - Information by UniProt
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Database links
- Entrez Gene: 10213 Human
- Entrez Gene: 59029 Mouse
- Entrez Gene: 311078 Rat
- Omim: 607173 Human
- SwissProt: O00487 Human
- SwissProt: O35593 Mouse
- Unigene: 567410 Human
- Unigene: 218198 Mouse
see all -
Alternative names
- 26S proteasome non-ATPase regulatory subunit 14 antibody
- 26S proteasome regulatory subunit rpn11 antibody
- 26S proteasome-associated PAD1 homolog 1 antibody
see all
Images
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Western blot - Anti-PSMD14 antibody [EPR4257] (ab109123)All lanes : Anti-PSMD14 antibody [EPR4257] (ab109123) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : A431 cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : Namalwa cell lysates
Lane 5 : K562 cell lysates
Lane 6 : MCF7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 35 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD14 antibody [EPR4257] (ab109123)](https://www.abcam.com/ps/products/109/ab109123/Images/ab109123-121714-anti-psmd14-antibody-epr4257-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD14 antibody [EPR4257] (ab109123)ab109123, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PSMD14 antibody [EPR4257] (ab109123)](https://www.abcam.com/ps/products/109/ab109123/Images/ab109123-121713-anti-psmd14-antibody-epr4257-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PSMD14 antibody [EPR4257] (ab109123)ab109123, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence. -
![Anti-PSMD14 antibody [EPR4257] (ab109123)](https://www.abcam.com/ps/products/109/ab109123/Images/ab109123-4-benefits-of-recombinant-antibodies.png)
Anti-PSMD14 antibody [EPR4257] (ab109123)
Protocols
References (0)
ab109123 has not yet been referenced specifically in any publications.
