Product nameAnti-PSMD14 antibody [EPR4257]
See all PSMD14 primary antibodies
DescriptionRabbit monoclonal [EPR4257] to PSMD14
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PSMD14 aa 150-250. The exact sequence is proprietary.
- HepG2, A431, HeLa, Namalwa, K562 and MCF7 cell lysates; Human colon tissue; HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 35 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min).|
|ICC/IF||1/500 - 1/1000.|
FunctionMetalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
Tissue specificityWidely expressed. Highest levels in heart and skeletal muscle.
Sequence similaritiesBelongs to the peptidase M67A family. PSMD14 subfamily.
Contains 1 MPN (JAB/Mov34) domain.
- Information by UniProt
- 26S proteasome non-ATPase regulatory subunit 14 antibody
- 26S proteasome regulatory subunit rpn11 antibody
- 26S proteasome-associated PAD1 homolog 1 antibody
All lanes : Anti-PSMD14 antibody [EPR4257] (ab109123) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : A431 cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : Namalwa cell lysates
Lane 5 : K562 cell lysates
Lane 6 : MCF7 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 35 kDa
ab109123, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab109123, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence.
ab109123 has not yet been referenced specifically in any publications.