Recombinant
RabMAb

Recombinant Anti-PSMD14 antibody [EPR4257] (ab109123)

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Overview

  • Product name

    Anti-PSMD14 antibody [EPR4257]
    See all PSMD14 primary antibodies

  • Description

    Rabbit monoclonal [EPR4257] to PSMD14

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PSMD14 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • HepG2, A431, HeLa, Namalwa, K562 and MCF7 cell lysates; Human colon tissue; HeLa cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min).
ICC/IF 1/500 - 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.

    • Tissue specificity

      Widely expressed. Highest levels in heart and skeletal muscle.

    • Sequence similarities

      Belongs to the peptidase M67A family. PSMD14 subfamily.
      Contains 1 MPN (JAB/Mov34) domain.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 26S proteasome non-ATPase regulatory subunit 14 antibody
      • 26S proteasome regulatory subunit rpn11 antibody
      • 26S proteasome-associated PAD1 homolog 1 antibody
      • 26S proteasome-associated PAD1 homolog antibody
      • PAD1 antibody
      • PAD1, yeast, homolog of antibody
      • POH1 antibody
      • Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody
      • Proteasome 26S subunit non ATPase 14 antibody
      • PSDE_HUMAN antibody
      • Psmd14 antibody
      • RPN11 antibody
      • Testis tissue sperm binding protein Li 69n antibody
      see all

    Images

    • Western blot - Anti-PSMD14 antibody [EPR4257] (ab109123)
      Western blot - Anti-PSMD14 antibody [EPR4257] (ab109123)
      All lanes : Anti-PSMD14 antibody [EPR4257] (ab109123) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : Namalwa cell lysates
      Lane 5 : K562 cell lysates
      Lane 6 : MCF7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD14 antibody [EPR4257] (ab109123)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMD14 antibody [EPR4257] (ab109123)

      ab109123, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-PSMD14 antibody [EPR4257] (ab109123)
      Immunocytochemistry/ Immunofluorescence - Anti-PSMD14 antibody [EPR4257] (ab109123)
      ab109123, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence.

    References

    ab109123 has not yet been referenced specifically in any publications.

    Publishing research using ab109123? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-PSMD14 antibody [EPR4257]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Purified protein (protein purified from yeast or recombinantly expre)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycine Biorad Stain free)
    Loading amount
    0.4 µg
    Specification
    protein purified from yeast or recombinantly expre
    Blocking step
    Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C
    Read More

    Ellen Goodall

    Verified customer

    Submitted Aug 01 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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