Overview

  • Product name

    Anti-PSMD14 antibody [EPR4258]
    See all PSMD14 primary antibodies
  • Description

    Rabbit monoclonal [EPR4258] to PSMD14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PSMD14 aa 250-350. The exact sequence is proprietary.

  • Positive control

    • HepG2, A431, K562, Namalwa, HeLa, and MCF7 cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
    • Tissue specificity

      Widely expressed. Highest levels in heart and skeletal muscle.
    • Sequence similarities

      Belongs to the peptidase M67A family. PSMD14 subfamily.
      Contains 1 MPN (JAB/Mov34) domain.
    • Information by UniProt
    • Database links

    • Alternative names

      • 26S proteasome non-ATPase regulatory subunit 14 antibody
      • 26S proteasome regulatory subunit rpn11 antibody
      • 26S proteasome-associated PAD1 homolog 1 antibody
      • 26S proteasome-associated PAD1 homolog antibody
      • PAD1 antibody
      • PAD1, yeast, homolog of antibody
      • POH1 antibody
      • Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody
      • Proteasome 26S subunit non ATPase 14 antibody
      • PSDE_HUMAN antibody
      • Psmd14 antibody
      • RPN11 antibody
      • Testis tissue sperm binding protein Li 69n antibody
      see all

    Images

    • All lanes : Anti-PSMD14 antibody [EPR4258] (ab109130) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : Namalwa cell lysate
      Lane 5 : HeLa cell lysate
      Lane 6 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 35 kDa

    References

    This product has been referenced in:

    • Fletcher AJ  et al. Trivalent RING Assembly on Retroviral Capsids Activates TRIM5 Ubiquitination and Innate Immune Signaling. Cell Host Microbe 24:761-775.e6 (2018). Read more (PubMed: 30503508) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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