Overview

  • Product name

  • Description

    Rabbit polyclonal to PSMD5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human PSMD5 aa 1-264.

  • Positive control

    • 293T, A431, Jurkat, Raji, Mouse brain cell lysates; Human breast carcinoma tissue.
  • General notes

     This product was previously labelled as Proteasome 26S S5

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a PSMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5.
  • Sequence similarities

    Belongs to the proteasome subunit S5B/HSM3 family.
  • Domain

    Rich in dileucine repeats, which have been implicated in trafficking of a variety of transmembrane proteins.
  • Information by UniProt
  • Database links

  • Alternative names

    • 26S protease subunit S5 basic antibody
    • 26S proteasome non-ATPase regulatory subunit 5 antibody
    • 26S proteasome subunit S5B antibody
    • Protease 26S, subunit 5B antibody
    • Proteasome (prosome, macropain) 26S subunit, non ATPase, 5 antibody
    • Proteasome 19S S5B antibody
    • Proteasome 26S subunit, non ATPase, 5 antibody
    • PSMD5 antibody
    • PSMD5_HUMAN antibody
    • S5B antibody
    see all

Images

  • Anti-PSMD5 antibody (ab137733) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 56 kDa



    7.5% SDS PAGE
  • Anti-PSMD5 antibody (ab137733) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 56 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling PSMD5 with ab137733 at 1/250 dilution.

References

ab137733 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137733.
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