Anti-PSMD5 antibody (ab226371)
Key features and details
- Rabbit polyclonal to PSMD5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PSMD5 antibody
See all PSMD5 primary antibodies -
Description
Rabbit polyclonal to PSMD5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Sheep, Rabbit, Horse, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Giant Panda -
Immunogen
Synthetic peptide within Human PSMD5 aa 325-375. The exact sequence is proprietary.
Database link: Q16401 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226371 was affinity purified using an epitope specific to PSMD5 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 51,56 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 51,56 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a PSMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5. -
Sequence similarities
Belongs to the proteasome subunit S5B/HSM3 family. -
Domain
Rich in dileucine repeats, which have been implicated in trafficking of a variety of transmembrane proteins. - Information by UniProt
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Database links
- Entrez Gene: 5711 Human
- Entrez Gene: 66998 Mouse
- Omim: 604452 Human
- SwissProt: Q16401 Human
- SwissProt: Q8BJY1 Mouse
- Unigene: 193725 Human
- Unigene: 270632 Mouse
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Alternative names
- 26S protease subunit S5 basic antibody
- 26S proteasome non-ATPase regulatory subunit 5 antibody
- 26S proteasome subunit S5B antibody
see all
Images
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All lanes : Anti-PSMD5 antibody (ab226371) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 51,56 kDa
Exposure time: 3 minutesCell lysates were prepared with NETN lysis buffer.
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PSMD5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226371 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226371 at 1 µg/ml.
Lane 1: ab226371 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226371 has not yet been referenced specifically in any publications.