Key features and details
- Rabbit polyclonal to PSMD5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PSMD5 antibody
See all PSMD5 primary antibodies
DescriptionRabbit polyclonal to PSMD5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Sheep, Rabbit, Horse, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Giant Panda
Synthetic peptide within Human PSMD5 aa 325-375. The exact sequence is proprietary.
Database link: Q16401
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
This product was previously labelled as Proteasome 26S S5
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226371 was affinity purified using an epitope specific to PSMD5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 51,56 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActs as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a PSMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5.
Sequence similaritiesBelongs to the proteasome subunit S5B/HSM3 family.
DomainRich in dileucine repeats, which have been implicated in trafficking of a variety of transmembrane proteins.
- Information by UniProt
- 26S protease subunit S5 basic antibody
- 26S proteasome non-ATPase regulatory subunit 5 antibody
- 26S proteasome subunit S5B antibody
All lanes : Anti-PSMD5 antibody (ab226371) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 51,56 kDa
Exposure time: 3 minutes
Cell lysates were prepared with NETN lysis buffer.
PSMD5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226371 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226371 at 1 µg/ml.
Lane 1: ab226371 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226371 has not yet been referenced specifically in any publications.