Anti-PSMD7/Mov34 antibody (ab11436)
Key features and details
- Rabbit polyclonal to PSMD7/Mov34
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PSMD7/Mov34 antibody
See all PSMD7/Mov34 primary antibodies -
Description
Rabbit polyclonal to PSMD7/Mov34 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Drosophila melanogaster, Zebrafish -
Immunogen
Synthetic peptide corresponding to Human PSMD7/Mov34 aa 55-66.
Sequence:AVPFDEDDKDDS
(Peptide available asab41795) -
Positive control
- WB: K-562, Hep G2, HeLa, Jurkat, HEK-293, U-2 OS and A549 cell lysates. ICC: Hep G2 cells. IHC-P: Human skin tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa. |
Target
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Function
Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. -
Sequence similarities
Belongs to the peptidase M67A family.
Contains 1 MPN (JAB/Mov34) domain. - Information by UniProt
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Database links
- Entrez Gene: 37894 Drosophila melanogaster
- Entrez Gene: 5713 Human
- Omim: 157970 Human
- SwissProt: P26270 Drosophila melanogaster
- SwissProt: P51665 Human
- Unigene: 770 Drosophila melanogaster
- Unigene: 440604 Human
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Alternative names
- 26S proteasome non ATPase regulatory subunit 7 antibody
- 26S proteasome non-ATPase regulatory subunit 7 antibody
- 26S proteasome regulatory subunit RPN8 antibody
see all
Images
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All lanes : Anti-PSMD7/Mov34 antibody (ab11436) at 1 µg/ml
Lane 1 : K-652 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : HEK-293 whole cell lysate
Lane 6 : U-2 OS whole cell lysate
Lane 7 : A549 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.25 µg/mlWestern blot analysis was performed on whole cell extracts (30 µg lysate). The blots were probed with ab11436 at 1 ug/ml and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (0.25 µg/mL, 1:4000 dilution). A 40 kDa band corresponding to PSMD7 was observed across the cell lines tested. In addition, a ~50 kDa band was observed, which could possibly represent the 26s Proteasome Subunit S5B. Known quantity of protein samples were electrophoresed and resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.
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IHC image of ab11436 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11436, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
ICC/IF image of ab11436 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11436, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot of PSDM7 in K562 cell lysate using ab11436.
Lane 1 is ab11436
Lane 2 is ab11436 pre-incubated with peptide
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab11436 has been referenced in 4 publications.
- Meul T et al. Mitochondrial Regulation of the 26S Proteasome. Cell Rep 32:108059 (2020). PubMed: 32846138
- Nixon B et al. Proteomic Profiling of Mouse Epididymosomes Reveals their Contributions to Post-testicular Sperm Maturation. Mol Cell Proteomics 18:S91-S108 (2019). PubMed: 30213844
- Zhou W et al. Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis. Biol Reprod 96:159-173 (2017). PubMed: 28395327
- Fredriksson Å et al. Effects of aging and reproduction on protein quality control in soma and gametes of Drosophila melanogaster. Aging Cell 11:634-43 (2012). PubMed: 22507075