Key features and details
- Rabbit polyclonal to PSMD7/Mov34
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PSMD7/Mov34 antibody
See all PSMD7/Mov34 primary antibodies
DescriptionRabbit polyclonal to PSMD7/Mov34
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
- 293T, HeLa, Jurkat and NIH 3T3 whole cell lysates.
This product was previously labelled as PSMD7
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab140428 was affinity purified using an epitope specific to PSMD7/Mov34 immobilized on solid support.
Our Abpromise guarantee covers the use of ab140428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 37 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActs as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Sequence similaritiesBelongs to the peptidase M67A family.
Contains 1 MPN (JAB/Mov34) domain.
- Information by UniProt
- 26S proteasome non ATPase regulatory subunit 7 antibody
- 26S proteasome non-ATPase regulatory subunit 7 antibody
- 26S proteasome regulatory subunit RPN8 antibody
Immunohistochemistry of human ovarian carcinoma using ab140428 at 1/1000. Detected using DAB.
Immunohistochemistry of human lung carcinoma using ab140428 at 1/1000. Counterstained with hematoxylin, detected using DAB.
All lanes : Anti-PSMD7/Mov34 antibody (ab140428) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 3 minutes
Detection of PSMD7/Mov34 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab140428 at 6 µg/mg lysate for IP and 1µg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab140428 has not yet been referenced specifically in any publications.