Overview

  • Product name
    Anti-PSMD8 antibody [p31-27]
    See all PSMD8 primary antibodies
  • Description
    Mouse monoclonal [p31-27] to PSMD8
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • HeLa cell cytosolic preparation.
  • General notes
    The hybridoma secreting the antibody to subunit PSMD8 was generated by fusion of splenocytes from Balb/c mice that had received repeated immunisation with recombinant human RSPMD8 protein. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.065% Sodium azide
  • Concentration information loading...
  • Purification notes
    Partially purified to remove any particulate or precipitated materials.
  • Clonality
    Monoclonal
  • Clone number
    p31-27
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 30 kDa.

Target

  • Function
    Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Necessary for activation of the CDC28 kinase.
  • Sequence similarities
    Belongs to the proteasome subunit S14 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26S proteasome non ATPase regulatory subunit 8 antibody
    • 26S proteasome non-ATPase regulatory subunit 8 antibody
    • 26S proteasome regulatory subunit p31 antibody
    • 26S proteasome regulatory subunit RPN12 antibody
    • 26S proteasome regulatory subunit S14 antibody
    • AAV38494 antibody
    • HIP6 antibody
    • HYPF antibody
    • MGC1660 antibody
    • Nin1p antibody
    • P31 antibody
    • Proteasome (prosome macropain) 26S subunit non ATPase 8 antibody
    • PSMD8 antibody
    • PSMD8_HUMAN antibody
    • Rpn12 antibody
    • S14 antibody
    see all

Images

  • Anti-PSMD8 antibody [p31-27] (ab22640) at 1/5000 dilution (Western blot of HeLa cell cytosolic preparation to identify S14 protein)
    Developed using the ECL technique.

    Observed band size: 30 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 minute

References

ab22640 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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