Key features and details
- Rabbit polyclonal to PSMD9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PSMD9 antibody
See all PSMD9 primary antibodies
DescriptionRabbit polyclonal to PSMD9
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein (His-T7-tag) corresponding to Human PSMD9 aa 2-223. (Expressed in E.coli).
SDEEARQSGGSSQAGVVTVSDVQELMRRKEEIEAQIKANYDVLESQKGIG MNEPLVDCEGYPRSDVDLYQVRTARHNIICLQNDHKAVMKQVEEALHQLH ARDKEKQARDMAEAHKEAM SRKLGQSESQGPPRAFAKVNSISPGSPASIAGLQVDDEIVEFGSVNTQNF QSLHNIGSVVQHSEGKPLNVTVIRRGEKHQLRLVPTRWAGKGLLGCNIIP LQR
Database link: O00233
- IHC-P: Human liver, liver cancer, glioma, lung cancer, breast cancer and prostate tissue. WB: Human liver lysate; Mouse spleen, heart and placenta lysate; A549 cell lysate; Recombinant human PSMD9 protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 25 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionActs as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.
Tissue specificityExpressed in all tissues tested, highly expressed in liver and kidney.
Sequence similaritiesBelongs to the proteasome subunit p27 family.
Contains 1 PDZ (DHR) domain.
- Information by UniProt
- 26S proteasome non ATPase regulatory subunit 9 antibody
- 26S proteasome non-ATPase regulatory subunit 9 antibody
- 26S proteasome regulatory subunit p27 antibody
All lanes : Anti-PSMD9 antibody (ab233154) at 3 µg/ml
Lane 1 : Human liver lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Mouse heart lysate
Lane 4 : A549 (Human lung carcinoma cell line) cell lysate
Lane 5 : Mouse placenta lysate
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
A549 (Human lung carcinoma cell line) cells stained for PSMD9 using ab233154 (green) at 20 µg/mL in ICC/IF analysis. FITC-labeled secondary antibody.
Formalin-fixed, paraffin-embedded human prostate tissue stained for PSMD9 using ab233154 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for PSMD9 using ab233154 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for PSMD9 using ab233154 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human glioma tissue stained for PSMD9 using ab233154 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver tissue stained for PSMD9 using ab233154 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for PSMD9 using ab233154 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Anti-PSMD9 antibody (ab233154) at 2 µg/ml + Recombinant human PSMD9 protein
Predicted band size: 25 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233154 has not yet been referenced specifically in any publications.