Overview

  • Product name

  • Description

    Rabbit polyclonal to PSMD9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (His-T7-tag) corresponding to Human PSMD9 aa 2-223. (Expressed in E.coli).
    Sequence:

    SDEEARQSGGSSQAGVVTVSDVQELMRRKEEIEAQIKANYDVLESQKGIG MNEPLVDCEGYPRSDVDLYQVRTARHNIICLQNDHKAVMKQVEEALHQLH ARDKEKQARDMAEAHKEAM SRKLGQSESQGPPRAFAKVNSISPGSPASIAGLQVDDEIVEFGSVNTQNF QSLHNIGSVVQHSEGKPLNVTVIRRGEKHQLRLVPTRWAGKGLLGCNIIP LQR


    Database link: O00233

  • Positive control

    • IHC-P: Human liver, liver cancer, glioma, lung cancer, breast cancer and prostate tissue. WB: Human liver lysate; Mouse spleen, heart and placenta lysate; A549 cell lysate; Recombinant human PSMD9 protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.
ICC/IF Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.
  • Tissue specificity

    Expressed in all tissues tested, highly expressed in liver and kidney.
  • Sequence similarities

    Belongs to the proteasome subunit p27 family.
    Contains 1 PDZ (DHR) domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 26S proteasome non ATPase regulatory subunit 9 antibody
    • 26S proteasome non-ATPase regulatory subunit 9 antibody
    • 26S proteasome regulatory subunit p27 antibody
    • Homolog of rat Bridge 1 antibody
    • MGC8644 antibody
    • p27 antibody
    • Proteasome (prosome macropain) 26S subunit non ATPase 9 antibody
    • Proteasome 26S non ATPase regulatory subunit 9 antibody
    • Proteasome 26S subunit non ATPase 9 antibody
    • PSMD 9 antibody
    • Psmd9 antibody
    • PSMD9_HUMAN antibody
    • Rpn4 antibody
    see all

Images

  • All lanes : Anti-PSMD9 antibody (ab233154) at 3 µg/ml

    Lane 1 : Human liver lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : A549 (Human lung carcinoma cell line) cell lysate
    Lane 5 : Mouse placenta lysate

    Secondary
    All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

    Predicted band size: 25 kDa

  • A549 (Human lung carcinoma cell line) cells stained for PSMD9 using ab233154 (green) at 20 µg/mL in ICC/IF analysis. FITC-labeled secondary antibody.

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for PSMD9 using ab233154 at 10 µg/ml in immunohistochemical analysis. DAB staining.

  • Formalin-fixed, paraffin-embedded human breast cancer tissue stained for PSMD9 using ab233154 at 10 µg/ml in immunohistochemical analysis. DAB staining.

  • Formalin-fixed, paraffin-embedded human lung cancer tissue stained for PSMD9 using ab233154 at 10 µg/ml in immunohistochemical analysis. DAB staining.

  • Formalin-fixed, paraffin-embedded human glioma tissue stained for PSMD9 using ab233154 at 10 µg/ml in immunohistochemical analysis. DAB staining.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for PSMD9 using ab233154 at 10 µg/ml in immunohistochemical analysis. DAB staining.

  • Formalin-fixed, paraffin-embedded human liver cancer tissue stained for PSMD9 using ab233154 at 10 µg/ml in immunohistochemical analysis. DAB staining.

  • Anti-PSMD9 antibody (ab233154) at 2 µg/ml + Recombinant human PSMD9 protein

    Predicted band size: 25 kDa

References

ab233154 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233154.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up