Recombinant Anti-PSMD9 antibody [EPR12947] (ab181998)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12947] to PSMD9
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PSMD9 antibody [EPR12947]
See all PSMD9 primary antibodies -
Description
Rabbit monoclonal [EPR12947] to PSMD9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, HeLa, HepG2 and 293 lysates; Human kidney tissue; HepG2 and HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12947 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 25 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/500.
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Flow Cyt (Intra) |
1/300.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 25 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/500. |
Flow Cyt (Intra)
1/300. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process. -
Tissue specificity
Expressed in all tissues tested, highly expressed in liver and kidney. -
Sequence similarities
Belongs to the proteasome subunit p27 family.
Contains 1 PDZ (DHR) domain. - Information by UniProt
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Database links
- Entrez Gene: 5715 Human
- Entrez Gene: 67151 Mouse
- Entrez Gene: 161475 Rat
- Omim: 603146 Human
- SwissProt: O00233 Human
- SwissProt: Q9CR00 Mouse
- SwissProt: Q9WTV5 Rat
- Unigene: 131151 Human
see all -
Alternative names
- 26S proteasome non ATPase regulatory subunit 9 antibody
- 26S proteasome non-ATPase regulatory subunit 9 antibody
- 26S proteasome regulatory subunit p27 antibody
see all
Images
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All lanes : Anti-PSMD9 antibody [EPR12947] (ab181998) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP at 1/1000 dilution
Predicted band size: 25 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PSMD9 with ab181998 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling PSMD9 with ab181998 at 1/500 dilution followed by Goat anti rabbit IgG (Dylight 555) secondary and counter-stained with DAPI (blue).
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Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PSMD9with ab181998 at 1/300 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti-rabbit IgG (FITC) at 1/150 dilution.
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Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PSMD9 with ab181998 at 1/500 dilution. A Goat anti rabbit IgG(Alexa Fluor® 555) was used as secondary antibody. Counterstained with DAPI (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181998 has not yet been referenced specifically in any publications.