Overview

  • Product name

    Anti-PSMD9 antibody [EPR12947]
    See all PSMD9 primary antibodies
  • Description

    Rabbit monoclonal [EPR12947] to PSMD9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PSMD9 aa 1-100. The exact sequence is proprietary.
    Database link: O00233

  • Positive control

    • Human fetal liver, HeLa, HepG2 and 293 lysates; Human kidney tissue; HepG2 and HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12947
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/500.
Flow Cyt 1/300.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.
  • Tissue specificity

    Expressed in all tissues tested, highly expressed in liver and kidney.
  • Sequence similarities

    Belongs to the proteasome subunit p27 family.
    Contains 1 PDZ (DHR) domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 26S proteasome non ATPase regulatory subunit 9 antibody
    • 26S proteasome non-ATPase regulatory subunit 9 antibody
    • 26S proteasome regulatory subunit p27 antibody
    • Homolog of rat Bridge 1 antibody
    • MGC8644 antibody
    • p27 antibody
    • Proteasome (prosome macropain) 26S subunit non ATPase 9 antibody
    • Proteasome 26S non ATPase regulatory subunit 9 antibody
    • Proteasome 26S subunit non ATPase 9 antibody
    • PSMD 9 antibody
    • Psmd9 antibody
    • PSMD9_HUMAN antibody
    • Rpn4 antibody
    see all

Images

  • All lanes : Anti-PSMD9 antibody [EPR12947] (ab181998) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L) -HRP at 1/1000 dilution

    Predicted band size: 25 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PSMD9 with ab181998 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling PSMD9 with ab181998 at 1/500 dilution followed by Goat anti rabbit IgG (Dylight 555) secondary and counter-stained with DAPI (blue).

  • Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PSMD9 with ab181998 at 1/300 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti-rabbit IgG (FITC) at 1/150 dilution.

  • Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PSMD9 with ab181998 at 1/500 dilution. A Goat anti rabbit IgG(Alexa Fluor® 555) was used as secondary antibody. Counterstained with DAPI (blue).

References

ab181998 has not yet been referenced specifically in any publications.

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