Product nameAnti-PSME1 antibody
See all PSME1 primary antibodies
DescriptionRabbit polyclonal to PSME1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Cynomolgus monkey
Recombinant full length protein corresponding to Human PSME1 aa 1-249.
MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALN EANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPP CGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGV AVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQL VHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY
Database link: Q06323-1
- WB: Mouse liver tissue lysate. Raji whole cell lysate. IHC-P: Human liver cancer and testis tissues. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab236877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 29 kDa.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionImplicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
Sequence similaritiesBelongs to the PA28 family.
- Information by UniProt
- 11S regulator complex alpha subunit antibody
- 11S regulator complex subunit alpha antibody
- 29 kD MCP activator subunit antibody
All lanes : Anti-PSME1 antibody (ab236877) at 1/500 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29 kDa
Paraffin-embedded human testis tissue stained for PSME1 using ab236877 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human liver cancer tissue stained for PSME1 using ab236877 at 1/100 dilution in immunohistochemical analysis.
PSME1 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate using ab236877 at 1/200 dilution.
Lane 1: Rabbit control IgG in Jurkat whole cell lysate.
Lane 2: ab236877 in Jurkat whole cell lysate.
Lane 3: Jurkat whole cell lysate (10 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at a 1/2000 dilution.
ab236877 has not yet been referenced specifically in any publications.