Key features and details
- Rabbit polyclonal to PSME1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PSME1 antibody
See all PSME1 primary antibodies
DescriptionRabbit polyclonal to PSME1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Cynomolgus monkey
Recombinant full length protein corresponding to Human PSME1 aa 1-249.
MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALN EANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPP CGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGV AVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQL VHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY
Database link: Q06323-1
- WB: Mouse liver tissue lysate. Raji whole cell lysate. IHC-P: Human liver cancer and testis tissues. IP: Jurkat whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab236877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 29 kDa.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionImplicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
Sequence similaritiesBelongs to the PA28 family.
- Information by UniProt
- 11S regulator complex alpha subunit antibody
- 11S regulator complex subunit alpha antibody
- 29 kD MCP activator subunit antibody
All lanes : Anti-PSME1 antibody (ab236877) at 1/500 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29 kDa
Paraffin-embedded human testis tissue stained for PSME1 using ab236877 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human liver cancer tissue stained for PSME1 using ab236877 at 1/100 dilution in immunohistochemical analysis.
PSME1 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate using ab236877 at 1/200 dilution.
Lane 1: Rabbit control IgG in Jurkat whole cell lysate.
Lane 2: ab236877 in Jurkat whole cell lysate.
Lane 3: Jurkat whole cell lysate (10 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at a 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236877 has not yet been referenced specifically in any publications.