Anti-PSME1 antibody (ab3333)

Submit a review Q&A (1)References (3)
Western blot - Anti-PSME1 antibody (ab3333)

    Key features and details

    • Rabbit polyclonal to PSME1
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

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    Anti-PSME1 antibody [EPR10968(B)] (ab155985)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-PSME1 antibody
      See all PSME1 primary antibodies

    • Description

      Rabbit polyclonal to PSME1

    • Host species

      Rabbit

    • Specificity

      Detects PSME1 (proteasome activator complex subunit 1).

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Cow, Pig, Cynomolgus monkey

    • Immunogen

      Synthetic peptide corresponding to Human PSME1 aa 5-22.
      Sequence:

      RVQPEAQAKVDVFREDLC


      (Peptide available as ab4949)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Rat brain extract.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3333 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa).

    By Western blot, this antibody detects a 28 kDa protein representing PSME1 in rat brain extract.
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa).

    By Western blot, this antibody detects a 28 kDa protein representing PSME1 in rat brain extract.

    Target

    • Function

      Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

    • Sequence similarities

      Belongs to the PA28 family.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 11S regulator complex alpha subunit antibody
      • 11S regulator complex subunit alpha antibody
      • 29 kD MCP activator subunit antibody
      • 29kD MCP activator subunit antibody
      • Activator of multicatalytic protease subunit 1 antibody
      • IFI5111 antibody
      • IGUP I-5111 antibody
      • Interferon gamma IEF SSP 5111 antibody
      • Interferon gamma inducible protein 5111 antibody
      • Interferon gamma up regulated I 5111 protein antibody
      • Interferon gamma up-regulated I-5111 protein antibody
      • MGC8628 antibody
      • PA28a antibody
      • PA28alpha antibody
      • Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) antibody
      • Proteasome activator 28 subunit alpha antibody
      • Proteasome activator complex subunit 1 antibody
      • Proteasome activator subunit 1 antibody
      • PSME1 antibody
      • PSME1_HUMAN antibody
      • REG-alpha antibody
      • REGalpha antibody
      see all

    Images

    • Western blot - Anti-PSME1 antibody (ab3333)
      Western blot - Anti-PSME1 antibody (ab3333)

      Western blot detection of PSME1 in Hepa 1 cell lysate using ab3333.

    References (3)

    Publishing research using ab3333? Please let us know so that we can cite the reference in this datasheet.

    ab3333 has been referenced in 3 publications.

    • Donnelly SK  et al. Rac3 regulates breast cancer invasion and metastasis by controlling adhesion and matrix degradation. J Cell Biol 216:4331-4349 (2017). PubMed: 29061650
    • Glogauer JE  et al. Neutrophils Increase Oral Squamous Cell Carcinoma Invasion through an Invadopodia-Dependent Pathway. Cancer Immunol Res 3:1218-26 (2015). PubMed: 26112922
    • Pla A  et al. TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain. Cell Death Dis 5:e1066 (2014). WB ; Mouse . PubMed: 24556681

    Customer reviews and Q&As

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    Question

    I would like to confirm that ab3333will detect human reg 1 alpha by western blot. Also if not do you have an antibody that will? Thank-you Lisa

    Read More
    Answer

    Not to the best of my knoweldge. To eliminate all question, I would perform a BLAST search on the immunizing peptide.

    Read More

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