Anti-PSME1 antibody (ab3333)

Submit a review Q&A (1)References (3)

Overview

  • Product name

    Anti-PSME1 antibody
    See all PSME1 primary antibodies

  • Description

    Rabbit polyclonal to PSME1

  • Host species

    Rabbit

  • Specificity

    Detects PSME1 (proteasome activator complex subunit 1).

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Hamster, Dog, Human
    Predicted to work with: Cow, Pig, Cynomolgus monkey

  • Immunogen

    Synthetic peptide corresponding to Human PSME1 aa 5-22.
    Sequence:

    RVQPEAQAKVDVFREDLC


    (Peptide available as ab4949)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Rat brain extract.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa).

By Western blot, this antibody detects a 28 kDa protein representing PSME1 in rat brain extract.

Target

  • Function

    Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

  • Sequence similarities

    Belongs to the PA28 family.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 11S regulator complex alpha subunit antibody
    • 11S regulator complex subunit alpha antibody
    • 29 kD MCP activator subunit antibody
    • 29kD MCP activator subunit antibody
    • Activator of multicatalytic protease subunit 1 antibody
    • IFI5111 antibody
    • IGUP I-5111 antibody
    • Interferon gamma IEF SSP 5111 antibody
    • Interferon gamma inducible protein 5111 antibody
    • Interferon gamma up regulated I 5111 protein antibody
    • Interferon gamma up-regulated I-5111 protein antibody
    • MGC8628 antibody
    • PA28a antibody
    • PA28alpha antibody
    • Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) antibody
    • Proteasome activator 28 subunit alpha antibody
    • Proteasome activator complex subunit 1 antibody
    • Proteasome activator subunit 1 antibody
    • PSME1 antibody
    • PSME1_HUMAN antibody
    • REG-alpha antibody
    • REGalpha antibody
    see all

Images

  • Western blot - Anti-PSME1 antibody (ab3333)
    Western blot - Anti-PSME1 antibody (ab3333)

    Western blot detection of PSME1 in Hepa 1 cell lysate using ab3333.

References

Publishing research using ab3333? Please let us know so that we can cite the reference in this datasheet.

ab3333 has been referenced in 3 publications.

  • Donnelly SK  et al. Rac3 regulates breast cancer invasion and metastasis by controlling adhesion and matrix degradation. J Cell Biol 216:4331-4349 (2017). PubMed: 29061650
  • Glogauer JE  et al. Neutrophils Increase Oral Squamous Cell Carcinoma Invasion through an Invadopodia-Dependent Pathway. Cancer Immunol Res 3:1218-26 (2015). PubMed: 26112922
  • Pla A  et al. TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain. Cell Death Dis 5:e1066 (2014). WB ; Mouse . PubMed: 24556681

Customer reviews and Q&As

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Question

I would like to confirm that ab3333will detect human reg 1 alpha by western blot. Also if not do you have an antibody that will? Thank-you Lisa

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Answer

Not to the best of my knoweldge. To eliminate all question, I would perform a BLAST search on the immunizing peptide.

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