Anti-PSME1 antibody (ab3333)
Key features and details
- Rabbit polyclonal to PSME1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PSME1 antibody
See all PSME1 primary antibodies -
Description
Rabbit polyclonal to PSME1 -
Host species
Rabbit -
Specificity
Detects PSME1 (proteasome activator complex subunit 1). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Cow, Pig, Cynomolgus monkey
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Immunogen
Synthetic peptide corresponding to Human PSME1 aa 5-22.
Sequence:RVQPEAQAKVDVFREDLC
(Peptide available asab4949) -
Positive control
- Rat brain extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa).
By Western blot, this antibody detects a 28 kDa protein representing PSME1 in rat brain extract. |
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa). By Western blot, this antibody detects a 28 kDa protein representing PSME1 in rat brain extract. |
Target
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Function
Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. -
Sequence similarities
Belongs to the PA28 family. - Information by UniProt
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Database links
- Entrez Gene: 510041 Cow
- Entrez Gene: 102141082 Cynomolgus monkey
- Entrez Gene: 19186 Mouse
- Entrez Gene: 397572 Pig
- SwissProt: Q4U5R3 Cow
- SwissProt: P58238 Cynomolgus monkey
- SwissProt: P97371 Mouse
- SwissProt: Q64L94 Pig
see all -
Alternative names
- 11S regulator complex alpha subunit antibody
- 11S regulator complex subunit alpha antibody
- 29 kD MCP activator subunit antibody
see all
Images
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Western blot - Anti-PSME1 antibody (ab3333)
Western blot detection of PSME1 in Hepa 1 cell lysate using ab3333.
Datasheets and documents
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Datasheet download
References (3)
ab3333 has been referenced in 3 publications.
- Donnelly SK et al. Rac3 regulates breast cancer invasion and metastasis by controlling adhesion and matrix degradation. J Cell Biol 216:4331-4349 (2017). PubMed: 29061650
- Glogauer JE et al. Neutrophils Increase Oral Squamous Cell Carcinoma Invasion through an Invadopodia-Dependent Pathway. Cancer Immunol Res 3:1218-26 (2015). PubMed: 26112922
- Pla A et al. TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain. Cell Death Dis 5:e1066 (2014). WB ; Mouse . PubMed: 24556681
