Recombinant
RabMAb

Recombinant Anti-PSME1 antibody [EPR10967(B)] (ab155091)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10967(B)] to PSME1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PSME1 antibody [EPR10967(B)]
    See all PSME1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10967(B)] to PSME1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PSME1. The exact sequence is proprietary.

  • Positive control

    • MCF7 and Jurkat lysates; MCF7 and HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10967(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
ICC/IF 1/500.

For unpurified use at 1/100 - 1/250. 
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

    • Sequence similarities

      Belongs to the PA28 family.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 11S regulator complex alpha subunit antibody
      • 11S regulator complex subunit alpha antibody
      • 29 kD MCP activator subunit antibody
      • 29kD MCP activator subunit antibody
      • Activator of multicatalytic protease subunit 1 antibody
      • IFI5111 antibody
      • IGUP I-5111 antibody
      • Interferon gamma IEF SSP 5111 antibody
      • Interferon gamma inducible protein 5111 antibody
      • Interferon gamma up regulated I 5111 protein antibody
      • Interferon gamma up-regulated I-5111 protein antibody
      • MGC8628 antibody
      • PA28a antibody
      • PA28alpha antibody
      • Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) antibody
      • Proteasome activator 28 subunit alpha antibody
      • Proteasome activator complex subunit 1 antibody
      • Proteasome activator subunit 1 antibody
      • PSME1 antibody
      • PSME1_HUMAN antibody
      • REG-alpha antibody
      • REGalpha antibody
      see all

    Images

    • Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
      Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
      All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 29 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
      Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)

      Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling PSME1 with purified ab155091 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
      Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
      Immunofluorescent analysis of MCF7 cells labeling PSME1 with ab155091 at 1/100 dilution.
    • Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
      Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)This image is courtesy of an anonymous Abreview
      All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

      All lanes : Human prostate cancer whole tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 29 kDa


      Exposure time: 2 minutes

      See Abreview

    References

    Publishing research using ab155091? Please let us know so that we can cite the reference in this datasheet.

    ab155091 has been referenced in 2 publications.

    • Jimenez-Guardeño JM  et al. Immunoproteasome activation enables human TRIM5a restriction of HIV-1. Nat Microbiol 4:933-940 (2019). PubMed: 30886358
    • Welk V  et al. Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes. J Biol Chem 291:13147-59 (2016). PubMed: 27129254

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PSME1 antibody [EPR10967(B)]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Glioblastoma cells)
    Permeabilization
    Yes - Triton X-100 0.§%
    Specification
    Glioblastoma cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 03 2019

    Western blot abreview for Anti-PSME1 antibody [EPR10967(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma cells))
    Gel Running Conditions
    Reduced Denaturing (4 - 12%)
    Loading amount
    50000 cells
    Specification
    Glioblastoma cells)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 03 2019

    Western blot abreview for Anti-PSME1 antibody [EPR10967(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    50 µg
    Specification
    293T cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 07 2018

    Western blot abreview for Anti-PSME1 antibody [EPR10967(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Sample
    Human Tissue lysate - whole (prostate)
    Specification
    prostate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 27 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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