Recombinant
RabMAb

Anti-PSME1 antibody [EPR10967(B)] (ab155091)

Abreviews (1) Submit a Question

Overview

  • Product name
    Anti-PSME1 antibody [EPR10967(B)]
    See all PSME1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10967(B)] to PSME1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PSME1. The exact sequence is proprietary.

  • Positive control
    • MCF7 and Jurkat lysates; MCF7 and HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10967(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
ICC/IF 1/500.

For unpurified use at 1/100 - 1/250. 
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function
      Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
    • Sequence similarities
      Belongs to the PA28 family.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • 11S regulator complex alpha subunit antibody
      • 11S regulator complex subunit alpha antibody
      • 29 kD MCP activator subunit antibody
      • 29kD MCP activator subunit antibody
      • Activator of multicatalytic protease subunit 1 antibody
      • IFI5111 antibody
      • IGUP I-5111 antibody
      • Interferon gamma IEF SSP 5111 antibody
      • Interferon gamma inducible protein 5111 antibody
      • Interferon gamma up regulated I 5111 protein antibody
      • Interferon gamma up-regulated I-5111 protein antibody
      • MGC8628 antibody
      • PA28a antibody
      • PA28alpha antibody
      • Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) antibody
      • Proteasome activator 28 subunit alpha antibody
      • Proteasome activator complex subunit 1 antibody
      • Proteasome activator subunit 1 antibody
      • PSME1 antibody
      • PSME1_HUMAN antibody
      • REG-alpha antibody
      • REGalpha antibody
      see all

    Images

    • Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
      Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
      All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 29 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
      Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)

      Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling PSME1 with purified ab155091 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
      Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
      Immunofluorescent analysis of MCF7 cells labeling PSME1 with ab155091 at 1/100 dilution.
    • Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)
      Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)This image is courtesy of an anonymous Abreview
      All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

      All lanes : Human prostate cancer whole tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 29 kDa


      Exposure time: 2 minutes

      See Abreview

    References

    ab155091 has not yet been referenced specifically in any publications.

    Publishing research using ab155091? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    1 Review or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-PSME1 antibody [EPR10967(B)]

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Sample
    Human Tissue lysate - whole (prostate)
    Specification
    prostate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Feb 27 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up