Anti-PSME1 antibody [EPR10967(B)] (ab155091)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-PSME1 antibody [EPR10967(B)]
See all PSME1 primary antibodies -
DescriptionRabbit monoclonal [EPR10967(B)] to PSME1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human PSME1. The exact sequence is proprietary.
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Positive control
- MCF7 and Jurkat lysates; MCF7 and HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10967(B)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 29 kDa. | |
| ICC/IF | 1/500. For unpurified use at 1/100 - 1/250. |
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| IP | 1/10 - 1/100. |
Target
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FunctionImplicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
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Sequence similaritiesBelongs to the PA28 family.
- Information by UniProt
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Database links
- Entrez Gene: 5720 Human
- Entrez Gene: 19186 Mouse
- Entrez Gene: 29630 Rat
- Omim: 600654 Human
- SwissProt: Q06323 Human
- SwissProt: P97371 Mouse
- SwissProt: Q63797 Rat
- Unigene: 75348 Human
see all -
Alternative names
- 11S regulator complex alpha subunit antibody
- 11S regulator complex subunit alpha antibody
- 29 kD MCP activator subunit antibody
see all
Images
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![Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)](https://www.abcam.com/ps/products/155/ab155091/Images/PSME1-Primary-antibodies-ab155091-1.jpg)
Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)](https://www.abcam.com/ps/products/155/ab155091/Images/ab155091-4-ab155091-Hela-500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling PSME1 with purified ab155091 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
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![Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)](https://www.abcam.com/ps/products/155/ab155091/Images/PSME1-Primary-antibodies-ab155091-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)Immunofluorescent analysis of MCF7 cells labeling PSME1 with ab155091 at 1/100 dilution. -
![Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)](https://www.abcam.com/ps/products/155/ab155091/Images/ab155091-219137-ab155091wb.jpg)
Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)This image is courtesy of an anonymous AbreviewAll lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution
All lanes : Human prostate cancer whole tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Exposure time: 2 minutes
Datasheets and documents
Protocols
References
This product has been referenced in:
- Welk V et al. Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes. J Biol Chem 291:13147-59 (2016). Read more (PubMed: 27129254) »
