Name: Anti-PSME1 antibody [EPR10967(B)]
Brand: RabMAb
SKU: ab155091
Rating: 5 (4 reviews)

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Indirect ELISA - Anti-PSME1 antibody [EPR10967(B)] (ab155091)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10967(B)] to PSME1
Suitable for: Indirect ELISA, WB, ICC/IF
Knockout validated
Reacts with: Human

Carrier Free

Product name

Anti-PSME1 antibody [EPR10967(B)]
See all PSME1 primary antibodies
Description

Rabbit monoclonal [EPR10967(B)] to PSME1
Host species

Rabbit
Tested applications

Suitable for: Indirect ELISA, WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Synthetic peptide within Human PSME1. The exact sequence is proprietary.
Positive control
- WB: HEK293T, MCF7 and Jurkat lysates. ICC: MCF7 and HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10967(B)
Isotype

IgG
Research areas

Alternative Versions
- Anti-PSME1 antibody [EPR10967(B)] - BSA and Azide free (ab249185)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human PSME1 knockout HEK-293T cell line (ab266192)
KO cell lysates
- Human PSME1 knockout HEK-293T cell lysate (ab257613)
Positive Controls
- Jurkat whole cell lysate (ab7899)
Recombinant Protein
- Recombinant human PSME1 protein (ab206168)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Indirect ELISA		Use at an assay dependent concentration. Use at 31.25 ng /ml
WB	(3)	1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
ICC/IF	(1)	1/500. For unpurified use at 1/100 - 1/250.

Notes
Indirect ELISA Use at an assay dependent concentration. Use at 31.25 ng /ml
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
ICC/IF 1/500. For unpurified use at 1/100 - 1/250.

Application notes

Is unsuitable for Flow Cyt or IHC-P.

Function

Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
Sequence similarities

Belongs to the PA28 family.
Target information above from: UniProt accession Q06323 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5720 Human
- Entrez Gene: 19186 Mouse
- Entrez Gene: 29630 Rat
- Omim: 600654 Human
- SwissProt: Q06323 Human
- SwissProt: P97371 Mouse
- SwissProt: Q63797 Rat
- Unigene: 75348 Human
- Unigene: 830 Mouse
- Unigene: 2742 Rat
see all
Alternative names
- 11S regulator complex alpha subunit antibody
- 11S regulator complex subunit alpha antibody
- 29 kD MCP activator subunit antibody
- 29kD MCP activator subunit antibody
- Activator of multicatalytic protease subunit 1 antibody
- IFI5111 antibody
- IGUP I-5111 antibody
- Interferon gamma IEF SSP 5111 antibody
- Interferon gamma inducible protein 5111 antibody
- Interferon gamma up regulated I 5111 protein antibody
- Interferon gamma up-regulated I-5111 protein antibody
- MGC8628 antibody
- PA28a antibody
- PA28alpha antibody
- Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha) antibody
- Proteasome activator 28 subunit alpha antibody
- Proteasome activator complex subunit 1 antibody
- Proteasome activator subunit 1 antibody
- PSME1 antibody
- PSME1_HUMAN antibody
- REG-alpha antibody
- REGalpha antibody
see all

Indirect ELISA - Anti-PSME1 antibody [EPR10967(B)] (ab155091)

Indirect ELISA analysis of Human Proteasome activator complex subunit 1 using ab155091 at 1000 ng/mL dilution and secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 2500 ng/mL dilution
Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)

All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PSME1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

Lanes 1-4: Merged signal (red and green). Green - ab155091 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

ab155091 Anti-PSME1 antibody [EPR10967(B)] was shown to specifically react with PSME1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266192 (knockout cell lysate ab257613) was used. Wild-type and PSME1 knockout samples were subjected to SDS-PAGE. ab155091 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)

Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling PSME1 with purified ab155091 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)

All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 29 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PSME1 antibody [EPR10967(B)] (ab155091)

Immunofluorescent analysis of MCF7 cells labeling PSME1 with ab155091 at 1/100 dilution.
Western blot - Anti-PSME1 antibody [EPR10967(B)] (ab155091)This image is courtesy of an anonymous Abreview

All lanes : Anti-PSME1 antibody [EPR10967(B)] (ab155091) at 1/1000 dilution

All lanes : Human prostate cancer whole tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 2 minutes
See Abreview
Anti-PSME1 antibody [EPR10967(B)] (ab155091)

Western blot protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab155091? Please let us know so that we can cite the reference in this datasheet.

ab155091 has been referenced in 2 publications.

Jimenez-Guardeño JM et al. Immunoproteasome activation enables human TRIM5a restriction of HIV-1. Nat Microbiol 4:933-940 (2019). PubMed: 30886358
Welk V et al. Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes. J Biol Chem 291:13147-59 (2016). PubMed: 27129254

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Glioblastoma cells)

Permeabilization

Yes - Triton X-100 0.§%

Specification

Glioblastoma cells

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Jun 03 2019

Application

Western blot

Sample

Human Cell lysate - whole cell (Glioblastoma cells))

Gel Running Conditions

Reduced Denaturing (4 - 12%)

Loading amount

50000 cells

Specification

Glioblastoma cells)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 03 2019

Application

Western blot

Sample

Human Cell lysate - whole cell (293T cells)

Gel Running Conditions

Reduced Denaturing (4-12% Gradient Gel)

Loading amount

50 µg

Specification

293T cells

Blocking step

Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 07 2018

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (4-15%)

Sample

Human Tissue lysate - whole (prostate)

Specification

prostate

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 27 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

KO cell lines

KO cell lysates

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (2)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-PSME1 antibody [EPR10967(B)]

Western blot abreview for Anti-PSME1 antibody [EPR10967(B)]

Western blot abreview for Anti-PSME1 antibody [EPR10967(B)]

Western blot abreview for Anti-PSME1 antibody [EPR10967(B)]