Recombinant Anti-PSME2 antibody [EPR14931] (ab183727)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14931] to PSME2
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt (Intra), Indirect ELISA
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PSME2 antibody [EPR14931]
See all PSME2 primary antibodies -
Description
Rabbit monoclonal [EPR14931] to PSME2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, Flow Cyt (Intra), Indirect ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, PC3. PC12, Neuro-2a, 4T1, and HepG2 whole cell lysates (ab7900); IHC-P: Human kidney clear cell carcinoma and stomach tissues; ICC/IF: HeLa and HepG2 cells. Flow Cyt (intra): HepG2 and Neuro-2a cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14931 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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ICC/IF |
1/500.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/200 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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Indirect ELISA |
Use a concentration of 0.002 - 1 µg/ml.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
ICC/IF
1/500. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/200 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Indirect ELISA
Use a concentration of 0.002 - 1 µg/ml. |
Target
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Function
Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. -
Sequence similarities
Belongs to the PA28 family. - Information by UniProt
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Database links
- Entrez Gene: 5721 Human
- Entrez Gene: 19188 Mouse
- Entrez Gene: 100362709 Rat
- Entrez Gene: 100364216 Rat
- Entrez Gene: 29614 Rat
- Omim: 602161 Human
- SwissProt: Q9UL46 Human
- SwissProt: P97372 Mouse
see all -
Alternative names
- 11S regulator complex beta subunit antibody
- 11S regulator complex subunit beta antibody
- Activator of multicatalytic protease subunit 2 antibody
see all
Images
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All lanes : Anti-PSME2 antibody [EPR14931] (ab183727) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lane 3 : 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaExposure times:
Lane 1: 3.25 seconds
Lane 2: 3 minutes
Blocking Buffer and concentration: 5% NFDM/TBST
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling PSME2 with ab183727 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling PSME2 with ab183727 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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All lanes : Anti-PSME2 antibody [EPR14931] (ab183727) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC3 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 27 kDa -
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling PSME2 with ab183727 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling PSME2 with ab183727 at 1/500 dilution, followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PSME2 with ab183727 at 1/500 dilution, followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling PSME2 with ab183727 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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ELISA analysis of Human PSME2 recombinant protein at 250 ng/mL with ab183727. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PSME2 with ab183727 at 1/210 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab183727 has been referenced in 4 publications.
- Wang X et al. Increased expression of PSME2 is associated with clear cell renal cell carcinoma invasion by regulating BNIP3‑mediated autophagy. Int J Oncol 59:N/A (2021). PubMed: 34779489
- Adwal A et al. Tradeoff between metabolic i-proteasome addiction and immune evasion in triple-negative breast cancer. Life Sci Alliance 3:N/A (2020). PubMed: 32423906
- Woodle ES et al. Proteasomal adaptations underlying carfilzomib-resistance in human bone marrow plasma cells. Am J Transplant 20:399-410 (2020). PubMed: 31595669
- Jimenez-Guardeño JM et al. Immunoproteasome activation enables human TRIM5a restriction of HIV-1. Nat Microbiol 4:933-940 (2019). PubMed: 30886358