Overview

  • Product name
  • Description
    Rabbit polyclonal to PSME3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Zebra finch, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 204-254 of Human PSME3 (NP_005780.2).

  • Positive control
    • 293T, HeLa, Jurkat and mouse NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab157157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 29 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a donut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation.
  • Sequence similarities
    Belongs to the PA28 family.
  • Domain
    The C-terminal sequences affect heptamer stability and proteasome affinity.
  • Post-translational
    modifications
    Phosphorylated by MAP3K3.
  • Cellular localization
    Nucleus. Cytoplasm. Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.
  • Information by UniProt
  • Database links
  • Alternative names
    • 11S regulator complex gamma subunit antibody
    • 11S regulator complex subunit gamma antibody
    • Activator of multicatalytic protease subunit 3 antibody
    • Ki antibody
    • Ki antigen antibody
    • Ki nuclear autoantigen antibody
    • Ki, PA28 gamma antibody
    • PA28 gamma antibody
    • PA28g antibody
    • PA28gamma antibody
    • Proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) antibody
    • Proteasome (prosome, macropain) activator subunit 3 antibody
    • Proteasome activator 28 gamma antibody
    • Proteasome activator 28 subunit gamma antibody
    • Proteasome activator complex subunit 3 antibody
    • Proteasome activator subunit 3 antibody
    • PSME3 antibody
    • PSME3_HUMAN antibody
    • REG GAMMA antibody
    • REG-gamma antibody
    see all

Images

  • ab157157 Immunoprecipitating PSME3 in human HEK293 whole cell lysate. 250µg of cell lysate was incubated with the undiluted primary antibody and matrix (Protein G) for 18 hours at 4°C. For western blotting ab157157 (1/1000) was used to confirm successful immunoprecipation.

    See Abreview

  • All lanes : Anti-PSME3 antibody (ab157157) at 1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 29 kDa


    Exposure time: 3 minutes
  • Detection of PMSE3 by Western Blot of Immunprecipitate.
    ab157157 at 1µg/ml labeling PMSE3 in 293T whole cell lysate immunoprecipitated using ab157157 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 minutes.

References

ab157157 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Total protein in input
250 µg

Abcam user community

Verified customer

Submitted Apr 22 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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