Anti-PSME3 antibody (ab157157)
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- References
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Overview
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Product nameAnti-PSME3 antibody
See all PSME3 primary antibodies -
DescriptionRabbit polyclonal to PSME3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Zebra finch, Xenopus tropicalis
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 204-254 of Human PSME3 (NP_005780.2).
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Positive control
- 293T, HeLa, Jurkat and mouse NIH 3T3 whole cell lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab157157 was affinity purified using an epitope specific to PSME3 immobilized on solid support.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab157157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2500. Predicted molecular weight: 29 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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FunctionSubunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a donut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation.
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Sequence similaritiesBelongs to the PA28 family.
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DomainThe C-terminal sequences affect heptamer stability and proteasome affinity.
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Post-translational
modificationsPhosphorylated by MAP3K3. -
Cellular localizationNucleus. Cytoplasm. Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.
- Information by UniProt
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Database links
- Entrez Gene: 420017 Chicken
- Entrez Gene: 740582 Chimpanzee
- Entrez Gene: 100336105 Cow
- Entrez Gene: 101131213 Gorilla
- Entrez Gene: 100065788 Horse
- Entrez Gene: 10197 Human
- Entrez Gene: 19192 Mouse
- Entrez Gene: 100171504 Orangutan
see all -
Alternative names
- 11S regulator complex gamma subunit antibody
- 11S regulator complex subunit gamma antibody
- Activator of multicatalytic protease subunit 3 antibody
see all
Images
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Immunoprecipitation - Anti-PSME3 antibody (ab157157)This image is courtesy of an anonymous Abreviewab157157 Immunoprecipitating PSME3 in human HEK293 whole cell lysate. 250µg of cell lysate was incubated with the undiluted primary antibody and matrix (Protein G) for 18 hours at 4°C. For western blotting ab157157 (1/1000) was used to confirm successful immunoprecipation.
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Western blot - Anti-PSME3 antibody (ab157157)All lanes : Anti-PSME3 antibody (ab157157) at 1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-PSME3 antibody (ab157157)Detection of PMSE3 by Western Blot of Immunprecipitate.
ab157157 at 1µg/ml labeling PMSE3 in 293T whole cell lysate immunoprecipitated using ab157157 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 minutes.
Datasheets and documents
References
ab157157 has not yet been referenced specifically in any publications.
