Anti-PSME3 antibody (ab157157)
Key features and details
- Rabbit polyclonal to PSME3
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PSME3 antibody
See all PSME3 primary antibodies -
Description
Rabbit polyclonal to PSME3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Zebra finch, Xenopus tropicalis
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 204-254 of Human PSME3 (NP_005780.2).
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Positive control
- 293T, HeLa, Jurkat and mouse NIH 3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab157157 was affinity purified using an epitope specific to PSME3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/2500. Predicted molecular weight: 29 kDa.
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| IP | (1) |
Use at 2-10 µg/mg of lysate.
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| Notes |
|---|
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WB
1/500 - 1/2500. Predicted molecular weight: 29 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a donut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. -
Sequence similarities
Belongs to the PA28 family. -
Domain
The C-terminal sequences affect heptamer stability and proteasome affinity. -
Post-translational
modificationsPhosphorylated by MAP3K3. -
Cellular localization
Nucleus. Cytoplasm. Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase. - Information by UniProt
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Database links
- Entrez Gene: 420017 Chicken
- Entrez Gene: 740582 Chimpanzee
- Entrez Gene: 100336105 Cow
- Entrez Gene: 101131213 Gorilla
- Entrez Gene: 100065788 Horse
- Entrez Gene: 10197 Human
- Entrez Gene: 19192 Mouse
- Entrez Gene: 100171504 Orangutan
see all -
Alternative names
- 11S regulator complex gamma subunit antibody
- 11S regulator complex subunit gamma antibody
- Activator of multicatalytic protease subunit 3 antibody
see all
Images
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Immunoprecipitation - Anti-PSME3 antibody (ab157157)This image is courtesy of an anonymous Abreview
ab157157 Immunoprecipitating PSME3 in human HEK293 whole cell lysate. 250µg of cell lysate was incubated with the undiluted primary antibody and matrix (Protein G) for 18 hours at 4°C. For western blotting ab157157 (1/1000) was used to confirm successful immunoprecipation.
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Western blot - Anti-PSME3 antibody (ab157157)All lanes : Anti-PSME3 antibody (ab157157) at 1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-PSME3 antibody (ab157157)Detection of PMSE3 by Western Blot of Immunprecipitate.
ab157157 at 1µg/ml labeling PMSE3 in 293T whole cell lysate immunoprecipitated using ab157157 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab157157 has been referenced in 3 publications.
- Fan J et al. CKIP-1 limits foam cell formation and inhibits atherosclerosis by promoting degradation of Oct-1 by REG?. Nat Commun 10:425 (2019). PubMed: 30683852
- Shu Y et al. MicroRNA-195-5p inhibitor prevents the development of osteoarthritis by targeting REG?. Mol Med Rep 19:4561-4568 (2019). PubMed: 30942447
- Jia Q et al. Fisetin, via CKIP-1/REG?, limits oxidized LDL-induced lipid accumulation and senescence in RAW264.7 macrophage-derived foam cells. Eur J Pharmacol 865:172748 (2019). PubMed: 31655030