Key features and details
- Rabbit polyclonal to PSME3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PSME3 antibody
See all PSME3 primary antibodies
DescriptionRabbit polyclonal to PSME3
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Pig, Cynomolgus monkey, Orangutan
Recombinant full length protein corresponding to Human PSME3 aa 1-254.
MASLLKVDQEVKLKVDSFRERITSEAEDLVANFFPKKLLELDSFLKEPIL NIHDLTQIHSDMNLPVPDPILLTNSHDGLDGPTYKKRRLDECEEAFQGTK VFVMPNGMLKSNQQLVDIIEKVKPEIRLLIEKCNTVKMWVQLLIPRIEDG NNFGVSIQEETVAELRTVESEAASYLDQISRYYITRAKLVSKIAKYPHVE DYRRTVTEIDEKEYISLRLIISELRNQYVTLHDMILKNIEKIKRPRSSNA ETLY
Database link: P61289
- MCF7 and PC12 cell lysates; Human colon cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab180829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 29 kDa.|
FunctionSubunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a donut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation.
Sequence similaritiesBelongs to the PA28 family.
DomainThe C-terminal sequences affect heptamer stability and proteasome affinity.
modificationsPhosphorylated by MAP3K3.
Cellular localizationNucleus. Cytoplasm. Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase.
- Information by UniProt
- 11S regulator complex gamma subunit antibody
- 11S regulator complex subunit gamma antibody
- Activator of multicatalytic protease subunit 3 antibody
All lanes : Anti-PSME3 antibody (ab180829) at 1/500 dilution
Lane 1 : MCF7 cell extract
Lane 2 : PC-12 cell extract
Predicted band size: 29 kDa
Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab180829. Blue DAPI for nuclear staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon cancer tissue labeling PSME3 with ab180829 at 1/50 dilution.
ab180829 has not yet been referenced specifically in any publications.