Key features and details
- Rabbit polyclonal to PSMF1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PSMF1 antibody
See all PSMF1 primary antibodies
DescriptionRabbit polyclonal to PSMF1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat
- 293T, HeLa, Jurkat whole cell lysate (ab7899)
This product was previously labelled as Proteasome Inhibitor subunit 1 PI3 1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab140497 was affinity purified using an epitope specific to PSMF1 immobilized on a solid support.
Our Abpromise guarantee covers the use of ab140497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 30 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPlays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
Sequence similaritiesBelongs to the proteasome inhibitor PI31 family.
- Information by UniProt
- hPI31 antibody
- PI31 antibody
- Proteasome (prosome macropain) inhibitor subunit 1 antibody
All lanes : Anti-PSMF1 antibody (ab140497) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 3 minutes
ab140497 at 1 µg/ml detecting PSMF1 in 293T whole cell lysate by WB following IP.
Lane 1: ab140497 at 6µg/mg of lysate
Lane 2: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab140497 has been referenced in 1 publication.
- Liu K et al. PI31 Is an Adaptor Protein for Proteasome Transport in Axons and Required for Synaptic Development. Dev Cell 50:509-524.e10 (2019). PubMed: 31327739