Overview

  • Product name

  • Description

    Rabbit polyclonal to PSMF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human PSMF1 aa 150-200.
    Database link: NP_848693.2

  • Positive control

    • 293T, HeLa and Jurkat whole cell lysates
  • General notes

     This product was previously labelled as Proteasome Inhibitor subunit 1 PI3 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab140527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.
  • Sequence similarities

    Belongs to the proteasome inhibitor PI31 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • hPI31 antibody
    • PI31 antibody
    • Proteasome (prosome macropain) inhibitor subunit 1 antibody
    • Proteasome inhibitor hP131 subunit antibody
    • Proteasome inhibitor PI31 subunit antibody
    • PSMB2 antibody
    • Psmf1 antibody
    • PSMF1_HUMAN antibody
    • RP23 402M7.5 antibody
    • RP4 545L17.1 antibody
    see all

Images

  • All lanes : Anti-PSMF1 antibody (ab140527) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Predicted band size: 30 kDa

  • Immunoprecipitation of PSMF1 from 293T whole cell lysate using ab140527 at 6 µg/mg lysate (1 mg for IP; 20% of IP loaded). For blotting immunoprecipitated PSMF1, ab140527 was used at 0.4 µg/ml.
    Detection: chemiluminescence with an exposure time of 10 seconds.

References

ab140527 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 26 2017

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