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RecombinantRabMAb

Recombinant Anti-Psoriasin antibody [EPR10433(2)] (ab172966)

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Western blot - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)
  • Immunoprecipitation - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)
  • Western blot - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)
  • Anti-Psoriasin antibody [EPR10433(2)] (ab172966)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10433(2)] to Psoriasin
  • Suitable for: WB, IP
  • Reacts with: Human, Recombinant fragment

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Recombinant Human Psoriasin protein (ab105577)
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Overview

  • Product name

    Anti-Psoriasin antibody [EPR10433(2)]
    See all Psoriasin primary antibodies
  • Description

    Rabbit monoclonal [EPR10433(2)] to Psoriasin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Human, Recombinant fragment
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Psoriasin aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P31151

  • Positive control

    • Human pancreas, A431 and MDA-MB435 lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10433(2)
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Signal transduction
    • Other
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Alternative Versions

    • Anti-Psoriasin antibody [EPR10433(2)] - BSA and Azide free (ab249738)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Psoriasin protein (ab105577)
  • Related Products

    • Recombinant Human Psoriasin protein (ab105577)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 11 kDa.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 11 kDa.
IP
1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Tissue specificity

      Fetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients.
    • Sequence similarities

      Belongs to the S-101 family.
      Contains 2 EF-hand domains.
    • Cellular localization

      Cytoplasm. Secreted. Secreted by a non-classical secretory pathway.
    • Target information above from: UniProt accession P31151 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6278 Human
      • Omim: 600353 Human
      • SwissProt: P31151 Human
      • Unigene: 112408 Human
      • Alternative names

        • HID 5 antibody
        • Protein S100 A7 antibody
        • Protein S100-A7 antibody
        • PSOR 1 antibody
        • PSOR1 antibody
        • Psoriasin 1 antibody
        • Psoriasin antibody
        • Psoriasin1 antibody
        • S100 Calcium binding protein A7 antibody
        • S100 calcium-binding protein A7 antibody
        • S100A7 antibody
        • S100A7c antibody
        • S10A7_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)
        Western blot - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)
        Lane 1 : Anti-Psoriasin antibody [EPR10433(2)] (ab172966) at 1/1000 dilution
        Lane 2 : Anti-Psoriasin antibody [EPR10433(2)] (ab172966) at 1/10000 dilution

        All lanes : full length recombinant protein tagged GST

        Predicted band size: 11 kDa
        Observed band size: 37 kDa why is the actual band size different from the predicted?

      • Immunoprecipitation - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)
        Immunoprecipitation - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)

        Western blot analysis on immunoprecipitation pellet from A431 cell lysate using ab172966.

      • Western blot - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)
        Western blot - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)
        All lanes : Anti-Psoriasin antibody [EPR10433(2)] (ab172966) at 1/1000 dilution

        Lane 1 : Human pancreas lysate
        Lane 2 : A431 cell lysate
        Lane 3 : MDA-MB-435 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 11 kDa

      • Anti-Psoriasin antibody [EPR10433(2)] (ab172966)
        Anti-Psoriasin antibody [EPR10433(2)] (ab172966)

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab172966? Please let us know so that we can cite the reference in this datasheet.

      ab172966 has not yet been referenced specifically in any publications.

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