Anti-Psoriasin antibody [EPR10433(2)] (ab172966)
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Overview
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Product nameAnti-Psoriasin antibody [EPR10433(2)]
See all Psoriasin primary antibodies -
DescriptionRabbit monoclonal [EPR10433(2)] to Psoriasin
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivityReacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Psoriasin aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P31151 -
Positive control
- Human pancreas, A431 and MDA-MB435 lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10433(2)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab172966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 11 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Tissue specificityFetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients.
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Sequence similaritiesBelongs to the S-101 family.
Contains 2 EF-hand domains. -
Cellular localizationCytoplasm. Secreted. Secreted by a non-classical secretory pathway.
- Information by UniProt
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Database links
- Entrez Gene: 6278 Human
- Omim: 600353 Human
- SwissProt: P31151 Human
- Unigene: 112408 Human
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Alternative names
- HID 5 antibody
- Protein S100 A7 antibody
- Protein S100-A7 antibody
see all
Images
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![Western blot - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)](https://www.abcam.com/ps/products/172/ab172966/Images/ab172966-249411-172966-WB.jpg)
Western blot - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)Lane 1 : Anti-Psoriasin antibody [EPR10433(2)] (ab172966) at 1/1000 dilution
Lane 2 : Anti-Psoriasin antibody [EPR10433(2)] (ab172966) at 1/10000 dilution
All lanes : full length recombinant protein tagged GST
Predicted band size: 11 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)](https://www.abcam.com/ps/products/172/ab172966/Images/ab172966-196315-ab172966IP.jpg)
Immunoprecipitation - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)Western blot analysis on immunoprecipitation pellet from A431 cell lysate using ab172966.
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![Western blot - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)](https://www.abcam.com/ps/products/172/ab172966/Images/ab172966-196312-wb1.jpg)
Western blot - Anti-Psoriasin antibody [EPR10433(2)] (ab172966)All lanes : Anti-Psoriasin antibody [EPR10433(2)] (ab172966) at 1/1000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : A431 cell lysate
Lane 3 : MDA-MB-435 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa
Datasheets and documents
References
ab172966 has not yet been referenced specifically in any publications.
