Key features and details
- Mouse monoclonal [1L4] to PSPC1 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-PSPC1 antibody [1L4] - C-terminal
See all PSPC1 primary antibodies
DescriptionMouse monoclonal [1L4] to PSPC1 - C-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Mouse PSPC1 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q8R326
- Hepa1-6 whole cell lysate; HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purification notesPurified Immunoglobulin
Our Abpromise guarantee covers the use of ab214012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 59 kDa.|
|ICC/IF||Use a concentration of 5 - 10 µg/ml.|
FunctionRegulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers.
Tissue specificityExpressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
Sequence similaritiesBelongs to the PSPC family.
Contains 2 RRM (RNA recognition motif) domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Nucleus matrix. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in a RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.
- Information by UniProt
- Paraspeckle component 1 antibody
- Paraspeckle protein 1 antibody
- PSP1 antibody
Lane 1 : Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 0.5 µg/ml
Lane 2 : Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 1 µg/ml
Lane 3 : Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 2 µg/ml
All lanes : Hepa1-6 whole cell lysate
All lanes : Goat Anti-Mouse IgG-Peroxidase
Developed using the ECL technique.
Predicted band size: 59 kDa
Immunofluorescent analysis of HeLa cells,4% paraformaldehyde fixed and 0.5% Triton X-100 permeabilized, labeling PSPC1 with ab214012 at 5 μg/mL dilution. Secondary antibody: Goat Anti-Mouse IgG, Cy3 conjugate.
ab214012 has not yet been referenced specifically in any publications.