Anti-PSPC1 antibody [1L4] - C-terminal (ab214012)

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Overview

  • Product name

    Anti-PSPC1 antibody [1L4] - C-terminal
    See all PSPC1 primary antibodies

  • Description

    Mouse monoclonal [1L4] to PSPC1 - C-terminal

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide within Mouse PSPC1 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8R326

  • Positive control

    • Hepa1-6 whole cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab214012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 59 kDa.
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • Function

    Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers.

  • Tissue specificity

    Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

  • Sequence similarities

    Belongs to the PSPC family.
    Contains 2 RRM (RNA recognition motif) domains.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus > nucleolus. Nucleus matrix. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in a RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Paraspeckle component 1 antibody
    • Paraspeckle protein 1 antibody
    • PSP1 antibody
    • Pspc1 antibody
    • PSPC1_HUMAN antibody
    see all

Images

  • Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012)
    Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012)
    Lane 1 : Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 0.5 µg/ml
    Lane 2 : Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 1 µg/ml
    Lane 3 : Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 2 µg/ml

    All lanes : Hepa1-6 whole cell lysate

    Secondary
    All lanes : Goat Anti-Mouse IgG-Peroxidase

    Developed using the ECL technique.

    Predicted band size: 59 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012)
    Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012)

    Immunofluorescent analysis of HeLa cells,4% paraformaldehyde fixed and 0.5% Triton X-100 permeabilized, labeling PSPC1 with ab214012 at 5 μg/mL dilution. Secondary antibody: Goat Anti-Mouse IgG, Cy3 conjugate.

References

ab214012 has not yet been referenced specifically in any publications.

Publishing research using ab214012? Please let us know so that we can cite the reference in this datasheet.

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