Anti-PSPC1 antibody [1L4] - C-terminal (ab214012)
![Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012)](https://www.abcam.com/ps/products/214/ab214012/Images/ab214012-265068-ab214012.jpg "Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012)")
Key features and details
- Mouse monoclonal [1L4] to PSPC1 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
-
Product name
Anti-PSPC1 antibody [1L4] - C-terminal
See all PSPC1 primary antibodies -
Description
Mouse monoclonal [1L4] to PSPC1 - C-terminal -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide within Mouse PSPC1 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q8R326 -
Positive control
- Hepa1-6 whole cell lysate; HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Purified Immunoglobulin -
Clonality
Monoclonal -
Clone number
1L4 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 59 kDa.
|
|
| ICC/IF |
Use a concentration of 5 - 10 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 59 kDa. |
|
ICC/IF
Use a concentration of 5 - 10 µg/ml. |
Target
-
Function
Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers. -
Tissue specificity
Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart. -
Sequence similarities
Belongs to the PSPC family.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleolus. Nucleus matrix. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in a RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps. - Information by UniProt
-
Database links
- Entrez Gene: 55269 Human
- Entrez Gene: 66645 Mouse
- Entrez Gene: 305910 Rat
- Omim: 612408 Human
- SwissProt: Q8WXF1 Human
- SwissProt: Q8R326 Mouse
- SwissProt: Q4KLH4 Rat
- Unigene: 213198 Human
see all -
Alternative names
- Paraspeckle component 1 antibody
- Paraspeckle protein 1 antibody
- PSP1 antibody
see all
Images
-
Western blot - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012)Lane 1 : Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 0.5 µg/ml
Lane 2 : Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 1 µg/ml
Lane 3 : Anti-PSPC1 antibody [1L4] - C-terminal (ab214012) at 2 µg/ml
All lanes : Hepa1-6 whole cell lysate
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
Developed using the ECL technique.
Predicted band size: 59 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012)](https://www.abcam.com/ps/products/214/ab214012/Images/ab214012-265069-ab214012b.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody [1L4] - C-terminal (ab214012)Immunofluorescent analysis of HeLa cells,4% paraformaldehyde fixed and 0.5% Triton X-100 permeabilized, labeling PSPC1 with ab214012 at 5 μg/mL dilution. Secondary antibody: Goat Anti-Mouse IgG, Cy3 conjugate.
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab214012 has not yet been referenced specifically in any publications.
