Anti-PSPC1 antibody (ab104238)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-PSPC1 antibody
See all PSPC1 primary antibodies -
DescriptionRabbit polyclonal to PSPC1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IPmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PSPC1.
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Positive control
- This antibody gave a positive signal in both HEK293 + U20S whole cell lysates and HepG2 and MCF7 cell lines in IF.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab104238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 58 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IP | Use a concentration of 5 µg/ml. |
Target
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FunctionRegulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers.
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Tissue specificityExpressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
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Sequence similaritiesBelongs to the PSPC family.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationNucleus > nucleolus. Nucleus matrix. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in a RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.
- Information by UniProt
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Database links
- Entrez Gene: 55269 Human
- Entrez Gene: 66645 Mouse
- Entrez Gene: 305910 Rat
- Omim: 612408 Human
- SwissProt: Q8WXF1 Human
- SwissProt: Q8R326 Mouse
- SwissProt: Q4KLH4 Rat
- Unigene: 213198 Human
see all -
Alternative names
- Paraspeckle component 1 antibody
- Paraspeckle protein 1 antibody
- PSP1 antibody
see all
Images
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Immunoprecipitation - Anti-PSPC1 antibody (ab104238)PSPC1 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104238.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 62kDa, non specific bands - 50 and 55kDa: We are unsure as to the identity of this extra band; PSPC1
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Western blot - Anti-PSPC1 antibody (ab104238)All lanes : Anti-PSPC1 antibody (ab104238) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 62 kDa (why is the actual band size different from the predicted?)
Exposure time: 2 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody (ab104238)ICC/IF image of ab104238 stained HepG2 cells. The cells were 100% methnaol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104238, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.
Protocols
References
This product has been referenced in:
- Wang Z et al. NEAT1 modulates herpes simplex virus-1 replication by regulating viral gene transcription. Cell Mol Life Sci 74:1117-1131 (2017). WB, IF . Read more (PubMed: 27783096) »
- Shen W et al. 2'-Fluoro-modified phosphorothioate oligonucleotide can cause rapid degradation of P54nrb and PSF. Nucleic Acids Res 43:4569-78 (2015). Read more (PubMed: 25855809) »
Customer reviews and Q&As
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