Product nameAnti-PSPC1 antibody
See all PSPC1 primary antibodies
DescriptionRabbit polyclonal to PSPC1
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human PSPC1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in both HEK293 + U20S whole cell lysates and HepG2 and MCF7 cell lines in IF.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab104238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 58 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IP||Use a concentration of 5 µg/ml.|
FunctionRegulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers.
Tissue specificityExpressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
Sequence similaritiesBelongs to the PSPC family.
Contains 2 RRM (RNA recognition motif) domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Nucleus matrix. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in a RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.
- Information by UniProt
- Paraspeckle component 1 antibody
- Paraspeckle protein 1 antibody
- PSP1 antibody
All lanes : Anti-PSPC1 antibody (ab104238) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
PSPC1 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104238.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 62kDa, non specific bands - 50 and 55kDa: We are unsure as to the identity of this extra band; PSPC1
ICC/IF image of ab104238 stained HepG2 cells. The cells were 100% methnaol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104238, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.
This product has been referenced in:
- Yu JSE et al. The FUS-DDIT3 Interactome in Myxoid Liposarcoma. Neoplasia 21:740-751 (2019). Read more (PubMed: 31220736) »
- Wang Z et al. NEAT1 modulates herpes simplex virus-1 replication by regulating viral gene transcription. Cell Mol Life Sci 74:1117-1131 (2017). WB, IF . Read more (PubMed: 27783096) »