Product nameAnti-PSPC1 antibody
See all PSPC1 primary antibodies
DescriptionRabbit polyclonal to PSPC1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Chimpanzee, Chinese hamster, Bat, Elephant
Synthetic peptide, corresponding to a region within amino acids 245-275 of Human PSPC1 (NP_001035879.1).
- HeLa, 293T or Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab115184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 59 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRegulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers.
Tissue specificityExpressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
Sequence similaritiesBelongs to the PSPC family.
Contains 2 RRM (RNA recognition motif) domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Nucleus matrix. Cytoplasm. In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in a RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.
- Information by UniProt
- Paraspeckle component 1 antibody
- Paraspeckle protein 1 antibody
- PSP1 antibody
All lanes : Anti-PSPC1 antibody (ab115184) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 59 kDa
Exposure time: 3 minutes
Samples: whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded).
Antibodies: ab115184 used for WB at 0.4 µg/ml and used for IP at 6 µg/mg lysate.
Detection: chemiluminescence with exposure time of 30 seconds (B).
ab115184 has been referenced in 1 publication.
- Wang J et al. RNA-binding protein PSPC1 promotes the differentiation-dependent nuclear export of adipocyte RNAs. J Clin Invest 127:987-1004 (2017). PubMed: 28192372